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Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis
Toxicology Letters ( IF 2.9 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.toxlet.2019.12.024
Lei Yang 1 , Suiqin Wang 2 , Guanghui Zhao 3 , Xi Wang 4 , Xiong Guo 4
Affiliation  

T-2 toxin and deoxynivalenol (DON) are two representative mycotoxins that are commonly found in cereals and agricultural products. As T-2 toxin and DON are considered the cause of Kashin-Beck disease, a special osteoarticular disease, chondrocytes would be a vital target site for these toxins. To fully understand the toxicity effects of T-2 toxin and DON on chondrocytes, the present study investigated and compared the gene expression profiles and underlying mechanisms of T-2 toxin and DON on cultured human chondrocytes by microarray and bioinformatics analysis. Normal human chondrocytes were treated with T-2 toxin at 0.01 μg/ml and DON at 1.0 μg/ml for 72 h and analyzed by microarray using Affymetrix Human Gene Chip. Comprehensive analysis, including gene ontology, pathways and gene-gene networks was performed to identify the crucial gene functions, related signal pathways and key genes. A total of 175 and 237 differentially expressed genes were identified in human chondrocytes for T-2 toxin and DON treatment, respectively. Of these, 47 had the same expression tendencies in the two groups. The protein-protein interaction network analysis showed that the 10 hub genes were different between the two groups. Our results provide a comprehensive understanding of the toxic mechanism of T-2 toxin and DON on human chondrocytes and suggest that although T-2 toxin and DON showed some similar toxic mechanisms in human chondrocytes, they also had different toxic characteristics.

中文翻译:

通过微阵列和生物信息学分析比较T-2毒素和脱氧雪腐镰刀菌烯醇对人软骨细胞的毒性机制

T-2 毒素和脱氧雪腐镰刀菌烯醇 (DON) 是谷物和农产品中常见的两种代表性真菌毒素。由于 T-2 毒素和 DON 被认为是大骨节病(一种特殊的骨关节疾病)的原因,因此软骨细胞将是这些毒素的重要靶点。为了充分了解 T-2 毒素和 DON 对软骨细胞的毒性作用,本研究通过微阵列和生物信息学分析研究和比较了 T-2 毒素和 DON 对培养的人软骨细胞的基因表达谱和潜在机制。正常人软骨细胞用 0.01 μg/ml 的 T-2 毒素和 1.0 μg/ml 的 DON 处理 72 小时,并使用 Affymetrix 人类基因芯片通过微阵列进行分析。综合分析,包括基因本体,进行通路和基因-基因网络以识别关键基因功能、相关信号通路和关键基因。在人类软骨细胞中分别鉴定出 175 和 237 个差异表达基因,分别用于 T-2 毒素和 DON 治疗。其中,47人在两组中具有相同的表达倾向。蛋白质-蛋白质相互作用网络分析表明,两组之间的10个枢纽基因不同。我们的研究结果全面了解了 T-2 毒素和 DON 对人软骨​​细胞的毒性机制,并表明虽然 T-2 毒素和 DON 在人软骨细胞中显示出一些相似的毒性机制,但它们也具有不同的毒性特征。在人类软骨细胞中分别鉴定出 175 和 237 个差异表达基因,分别用于 T-2 毒素和 DON 治疗。其中,47人在两组中具有相同的表达倾向。蛋白质-蛋白质相互作用网络分析表明,两组之间的10个枢纽基因不同。我们的研究结果全面了解了 T-2 毒素和 DON 对人软骨​​细胞的毒性机制,并表明虽然 T-2 毒素和 DON 在人软骨细胞中显示出一些相似的毒性机制,但它们也具有不同的毒性特征。在人类软骨细胞中分别鉴定出 175 和 237 个差异表达基因,分别用于 T-2 毒素和 DON 治疗。其中,47人在两组中具有相同的表达倾向。蛋白质-蛋白质相互作用网络分析表明,两组之间的10个枢纽基因不同。我们的研究结果全面了解了 T-2 毒素和 DON 对人软骨​​细胞的毒性机制,并表明虽然 T-2 毒素和 DON 在人软骨细胞中显示出一些相似的毒性机制,但它们也具有不同的毒性特征。
更新日期:2020-03-01
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