Primer design is essential to conduct whole plasmid site-directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time-consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition and percentage of guanine-cytosine (GC content). The running results showed that the script is able to search primers with mutations of target residue automatically. This script may facilitate primer design for whole plasmid site-directed mutagenesis and aid protein mutant construction.

中文翻译：

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用于设计定点诱变引物的python脚本。

引物设计对于进行整个质粒定点诱变进行蛋白质研究至关重要。传统上，诱变引物是手动设计的，既耗时又容易出错。在这里，我们介绍了一个Python脚本，用于通过预设核苷酸组成和鸟嘌呤-胞嘧啶百分比（GC含量）的参数来搜索引物。运行结果表明，该脚本能够自动搜索具有目标残基突变的引物。该脚本可能有助于整个质粒定点诱变的引物设计，并有助于蛋白质突变体的构建。