The selective and sensitive detection of biothiols; cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions is of considerable importance because of their pivotal roles in maintaining the reducing environment in the cells. This study describes a strategy for the determination of biothiols based on the PDI/Met‐Hg²⁺complex platform. We designed and fabricated methionine modified perylene diimide molecule as a selective sensing probe for Hg²⁺ ions in aqueous solutions (PDI/Met‐Hg²⁺). The complex between perylene bisimide derivative (PDI/Met) and Hg²⁺ was investigated and it demonstrated turn‐on fluorescence response for the detection of the biological thiols. Besides, PDI/Met displayed fluorescence quenching response in the presence of mercury ions and the emission intensity of PDI/Met‐Hg²⁺ was recovered after transferring biothiols (Cys, Hcy, and GSH). Thus, PDI/Met could be utilized as a fluorescent chemosensor for the sequential recognition of mercury ions and biological thiols.

中文翻译：

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基于Per衍生物的Hg2 +离子和生物硫醇的新型荧光探针及其在活细胞成像和理论计算中的应用

选择性和灵敏地检测生物硫醇；水溶液中的半胱氨酸（Cys），高半胱氨酸（Hcy）和谷胱甘肽（GSH）具有相当重要的意义，因为它们在维持细胞还原环境中起着关键作用。这项研究描述了一种基于PDI / Met-Hg ²⁺复杂平台的生物硫醇测定策略。我们设计和制造了蛋氨酸修饰的per二酰亚胺分子，作为水溶液中Hg ²⁺离子（PDI / Met-Hg ²⁺）的选择性传感探针。研究了per双酰亚胺衍生物（PDI / Met）与Hg ²⁺之间的络合物，并证明了用于检测生物硫醇的开启荧光响应。除了，PDI / Met在存在汞离子的情况下显示出荧光猝灭响应，转移生物硫醇（Cys，Hcy和GSH）后，PDI / Met-Hg ²⁺的发射强度得以恢复。因此，PDI / Met可用作荧光化学传感器，用于顺序识别汞离子和生物硫醇。