This study investigated the impact of dietary fiber from sweet potato residue (SPDF) on the diversity of the gut microbiota. An in vitro batch culture system simulating the human gut was used to understand the prebiotic role of SPDF. The results showed that SPDF mediated a significant increase in the concentrations of Bifidobacterium and Lactobacillus, whereas induced a significant decrease of Enterobacillus, Clostridium perfringens and Bacteroides. The prebiotic index and Bifidobacterium/Enterobacillus value were also significantly increased in SPDF groups compared to those of the control group, suggesting that SPDF had prebiotic effects. Furthermore, to investigate the effects of SPDF on the intestinal microecosystem, diets containing different concentrations of SPDF were used to feed Wistar rats for 4 weeks. 16S rRNA gene sequencing, short chain fatty acid quantification and physiochemical property analysis in the rat feces were then conducted. The results showed that SPDF significantly increased the Bacteroidetes to Firmicutes ratio at the phylum level and the amount of Akkermansia was also increased at the genus level, which was confirmed by qRT-PCR. The production of propionate and butyrate in the rat feces of both 3% and 15% SPDF groups was higher than that in the control group, which was further confirmed by the decrease of pH. Additionally, SPDF supplementation in this study resulted in a higher villus height to fossa depth ratio, which indicated improved digestion and absorption in the GI tract. Our findings support the utilization of SPDF from sweet potato residue in the development of potentially prebiotic food products for improving intestinal health.

中文翻译：

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从甘薯残留物中分离出的膳食纤维可促进健康的肠道微生物组特性。

这项研究调查了甘薯渣中膳食纤维对肠道菌群多样性的影响。使用模拟人肠道的体外分批培养系统来了解SPDF的益生元作用。结果表明，SPDF介导了双歧杆菌和乳杆菌浓度的显着增加，而引起肠杆菌，产气荚膜梭状芽胞杆菌和拟杆菌的浓度显着降低。与对照组相比，SPDF组的益生元指数和双歧杆菌/肠杆菌值也显着增加，表明SPDF具有益生元作用。此外，为了研究SPDF对肠道微生态系统的影响，使用含有不同浓度SPDF的饮食喂养Wistar大鼠4周。16S rRNA基因测序，然后在大鼠粪便中进行短链脂肪酸定量和理化性质分析。结果表明，SPDF在门类水平显着提高了拟杆菌的比率，在属水平上，阿克曼的含量也有所增加，这已通过qRT-PCR证实。SPDF组3％和15％的大鼠粪便中丙酸和丁酸的产量均高于对照组，这通过pH值的降低进一步得到证实。此外，本研究中补充SPDF导致绒毛高度与窝深度之比更高，这表明胃肠道的消化吸收得到改善。我们的研究结果支持在开发潜在的益生元食品以改善肠道健康方面利用甘薯渣中的SPDF。