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Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways.
Food & Function ( IF 5.1 ) Pub Date : 2020-01-02 , DOI: 10.1039/c9fo02322d Haibo He 1 , Minlu Feng , Haiyan Xu , Xiaomei Li , Yumin He , Huilin Qin , Yongfeng Zhang , Hongbo Tang , Kun Zou
Food & Function ( IF 5.1 ) Pub Date : 2020-01-02 , DOI: 10.1039/c9fo02322d Haibo He 1 , Minlu Feng , Haiyan Xu , Xiaomei Li , Yumin He , Huilin Qin , Yongfeng Zhang , Hongbo Tang , Kun Zou
Affiliation
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Our previous studies have demonstrated that the total triterpenes from the fruits of Chaenomeles speciosa (CSTT) exhibit effective therapeutic effects on gastric ulcer patients and animals. The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameliorate IND-induced gastric injury, which was related to promoting IND-damaged GES-1 cell proliferation and migration, improving the IND-damaged rat GBF, ulcer area, inhibition rate and pathologic changes of gastric mucous tissue, increasing the amount of adhered gastric mucus, attenuating the volume and total acidity of the gastric effluents, and augmenting the gastric pH; further studies showed that CSTT obviously downregulated miR-423-5p mRNA, NAG-1 mRNA and protein expression, Bax, Bad, cytosol cytochrome C, Apaf-1, cleaved-caspase-3, and cleaved-caspase-9 protein expression and cytosol cytochrome C concentration, and upregulated TFF1, TFF2 and TFF3 mRNA and protein expression, Bcl-2, Bcl-xl, pro-caspase-3, and pro-caspase-9 protein expression, mitochondrial viability, mitochondrial cytochrome C concentration and Bcl-2/Bax, Bcl-xl/Bad ratios. These findings demonstrated that CSTT protected against IND-induced gastric damage by depressing miR-423-5p expression and modulating the TFF/NAG-1 pathway, which in turn restrained mitochondrion-mediated apoptosis.
中文翻译：
木瓜果实中的总三萜类化合物通过调节microRNA-423-5p介导的TFF / NAG-1和细胞凋亡途径对消炎痛诱导的胃部损伤具有胃保护作用。
我们以前的研究表明，木瓜木瓜果实（CSTT）的总三萜烯对胃溃疡患者和动物具有有效的治疗作用。当前的目的是进一步研究所涉及的机制。结果表明，CSTT可以减轻IND引起的胃损伤，这与促进IND损伤的GES-1细胞的增殖和迁移，改善IND损伤的大鼠GBF，溃疡面积，抑制率和胃黏膜组织病理改变有关，增加胃粘液的黏附量，减弱胃液的体积和总酸度，并增加胃液的pH值；进一步的研究表明CSTT明显下调了miR-423-5p mRNA，NAG-1 mRNA和蛋白表达，Bax，Bad，细胞质细胞色素C，Apaf-1，裂解caspase-3，和裂解的caspase-9蛋白表达和胞浆细胞色素C浓度，并上调TFF1，TFF2和TFF3 mRNA和蛋白表达，Bcl-2，Bcl-xl，pro-caspase-3和pro-caspase-9蛋白表达，线粒体活力，线粒体细胞色素C浓度和Bcl-2 / Bax，Bcl-xl / Bad比。这些发现表明，CSTT通过抑制miR-423-5p表达和调节TFF / NAG-1途径来保护细胞免受IND诱导的胃损伤，从而抑制线粒体介导的细胞凋亡。
更新日期：2020-02-13
中文翻译：
木瓜果实中的总三萜类化合物通过调节microRNA-423-5p介导的TFF / NAG-1和细胞凋亡途径对消炎痛诱导的胃部损伤具有胃保护作用。
我们以前的研究表明，木瓜木瓜果实（CSTT）的总三萜烯对胃溃疡患者和动物具有有效的治疗作用。当前的目的是进一步研究所涉及的机制。结果表明，CSTT可以减轻IND引起的胃损伤，这与促进IND损伤的GES-1细胞的增殖和迁移，改善IND损伤的大鼠GBF，溃疡面积，抑制率和胃黏膜组织病理改变有关，增加胃粘液的黏附量，减弱胃液的体积和总酸度，并增加胃液的pH值；进一步的研究表明CSTT明显下调了miR-423-5p mRNA，NAG-1 mRNA和蛋白表达，Bax，Bad，细胞质细胞色素C，Apaf-1，裂解caspase-3，和裂解的caspase-9蛋白表达和胞浆细胞色素C浓度，并上调TFF1，TFF2和TFF3 mRNA和蛋白表达，Bcl-2，Bcl-xl，pro-caspase-3和pro-caspase-9蛋白表达，线粒体活力，线粒体细胞色素C浓度和Bcl-2 / Bax，Bcl-xl / Bad比。这些发现表明，CSTT通过抑制miR-423-5p表达和调节TFF / NAG-1途径来保护细胞免受IND诱导的胃损伤，从而抑制线粒体介导的细胞凋亡。
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