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A Homemade Smart Phone Microscope for Single-Particle Fluorescence Microscopy
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2019-12-19 , DOI: 10.1021/acs.jchemed.9b00670
Travis Varra 1 , Amy Simpson 1 , Benton Roesler 1 , Zach Nilsson 1 , Duncan Ryan 1 , Michael Van Erdewyk 1 , Jennifer D. Schuttlefield Christus 2 , Justin B. Sambur 1
Affiliation  

Imaging tools advance nanoscience education by enabling students to see and engage with matter that is not visible to the human eye. However, undergraduate students generally lack access to expensive research-grade electron and optical microscopes, especially at primarily undergraduate institutions. New, inexpensive, and hands-on microscopy activities are needed to advance nanoscience education. Here we demonstrate a microscopy activity in which students assemble a total internal reflection-based fluorescence microscope using interlocking building bricks and image a series of micrometer- and nanometer-sized fluorescent beads. The hands-on experiments are accompanied by fluorescence image analysis, single-particle detection, and particle size measurements. The activity enables students to explore the optical diffraction limit effect that was the subject of the 2014 Nobel Prize in Chemistry. This activity introduces undergraduate students to underlying principles of optical microscopy, optical components and light sources, Snell’s law, and image processing and analysis procedures while reinforcing major concepts like data analysis and statistics, fluorescence, emission, and absorption.

中文翻译:

用于单粒子荧光显微镜的自制智能手机显微镜

成像工具使学生能够看到并参与人眼看不到的物质,从​​而推动了纳米科学教育的发展。但是,本科生通常无法获得昂贵的研究级电子显微镜和光学显微镜,尤其是在主要的本科院校中。需要新的,廉价的和动手显微镜活动来推进纳米科学教育。在这里,我们展示了一个显微镜活动,学生可以使用互锁的积木组装一个基于全内反射的荧光显微镜,并对一系列微米和纳米尺寸的荧光珠成像。动手实验伴随着荧光图像分析,单颗粒检测和粒度测量。该活动使学生能够探索光学衍射极限效应,该效应是2014年诺贝尔化学奖的主题。该活动向大学生介绍光学显微镜的基本原理,光学组件和光源,斯涅尔定律以及图像处理和分析程序,同时加强诸如数据分析和统计,荧光,发射和吸收等主要概念。
更新日期:2019-12-19
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