4'-Ethynyl-2-fluoro-2'-deoxyadenosine (EFdA) and 4'-ethynyl-2'-deoxyadenosine (EdA) are nucleoside analogues which inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase. EdAP, a cyclosaligenyl (cycloSal) phosphate derivative of EdA, inhibits the replication of the influenza A virus. The common structural feature of these compounds is the ethynyl group at the 4'-position. In this study, these nucleoside analogues were prepared by a common synthetic strategy starting from the known 1,2-di-O-acetyl-D-ribofuranose. Biological evaluation of EdAP revealed that this compound reduced hepatitis B virus (HBV) replication dose-dependently without cytotoxicity against host cells tested in this study.

中文翻译：

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核苷酸类似物EFdA，EdA和EdAP的合成，以及EdAP对乙型肝炎病毒复制的影响。

4′-乙炔基-2-氟-2′-脱氧腺苷（EFdA）和4′-乙炔基-2′-脱氧腺苷（EdA）是抑制人免疫缺陷病毒1型（HIV-1）逆转录酶的核苷类似物。EdAP，EdA的磷酸环saligenyl（cycloSal）衍生物，可抑制甲型流感病毒的复制。这些化合物的共同结构特征是在4'-位的乙炔基。在这项研究中，这些核苷类似物是通过一种常见的合成策略从已知的1,2-二-O-乙酰基-D-呋喃核糖开始制备的。EdAP的生物学评估表明，该化合物可剂量依赖性地减少乙型肝炎病毒（HBV）复制，而对本研究中测试的宿主细胞无细胞毒性。