In the longevity research by using yeasts, chronological lifespan is defined as the survival time after entry into stationary phase. Previously, screening for long lived mutants of Schizosaccharomyces pombe was performed to identify the novel factors involved in longevity. From this screening, one long lived mutant called as No.36 was obtained. In this study, we identified the mutation caused in gas1+, which encodes glucanosyltransferase (gas1-287 mutation) is responsible for the longevity of No.36 mutant. Through the analysis of this mutant, we found that cell wall perturbing agent micafungin also extends chronological lifespan in fission yeast. This lifespan extension depended on both Pmk1 and Sty1 MAP kinases, and longevity caused by the gas1-287 mutation also depended on these kinases. In summary, we propose that the gas1-287 mutation causes longevity as the similar mechanism as cell wall stress depending on Pmk1 and Sty1 MAPK pathways.

中文翻译：

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gas1突变通过粟酒裂殖酵母中的Pmk1和Sty1 MAPKs延长了时间寿命。

在使用酵母进行的寿命研究中，按时间顺序将寿命定义为进入稳定期后的生存时间。以前，对粟酒裂殖酵母的长寿命突变体进行了筛选，以鉴定参与长寿的新因素。通过该筛选，获得了一种长寿命的突变体，称为No.36。在这项研究中，我们确定了在gas1 +中引起的突变，该突变编码glucanosyltransferase（gas1-287突变）是No.36突变体长寿的原因。通过对该突变体的分析，我们发现细胞壁扰动剂米卡芬净也延长了裂变酵母的时间寿命。这种寿命的延长取决于Pmk1和Sty1 MAP激酶，并且由gas1-287突变引起的寿命也取决于这些激酶。总之，