Although paraoxonase-1 (PON1) activity has been demonstrated to be a reliable biomarker of various diseases, clinical studies have been based only on relative comparison of specific enzyme activities, which capture differences mainly due to (usually unknown) PON1 concentration. Hence, the aim of this report is to present for the first time the simple evaluation method for determining autonomous kinetic parameter of PON1 that could be also associated with polymorphic forms and diseases; i.e. the Michaelis constant which is enzyme concentration independent quantity. This alternative approach significantly reduces the number of experiments needed, and it yields the results with great accuracy.

中文翻译：

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通过进度曲线的非线性拟合评估内酯酶活性的对氧磷酶-1动力学参数。

尽管已证明对氧磷酶-1（PON1）活性是各种疾病的可靠生物标志物，但临床研究仅基于特定酶活性的相对比较，该相对活性主要是由于（通常未知）PON1浓度引起的。因此，本报告的目的是首次提出一种简单的评估方法，以确定PON1的自主动力学参数，该参数也可能与多态性形式和疾病有关。即米氏常数，它是与酶浓度无关的量。这种替代方法大大减少了所需的实验次数，并且可以非常准确地得出结果。