Ethanol use is widespread in adolescents, yet only some transition to problematic drinking. It is important to understand why the risk for problematic drinking varies across sub-groups of adolescents. This study reports a short-term selection program to generate Wistar rat lines (high and low adolescent ethanol drinking, ADHI and ADLO lines, respectively) that significantly differ in ethanol drinking at adolescence. The S0 generation and filial generations 1 (S1), S2, and S3 of ADHI and ADLO offspring were tested for basal or stress-induced ethanol intake at adulthood, or for shelter-seeking and risk-taking in the multivariate concentric square field test (MSCF). The study generated lines with significant differences in free-choice ethanol drinking at adolescence. The effects of the selection were observed at adulthood, beyond the stage in which the selection was conducted: S1-ADHI but not S1-ADLO adult male rats exhibited stress-induced drinking. These effects were associated with significant alterations in shelter-seeking and risk-taking behaviors. ADHI rats spent significantly less time in areas of the MSCF whose exploration entails risk-taking and significantly more time in dark, sheltered areas. Some of these effects were normalized by the administration of 0.5 g/kg ethanol. There were no line differences in ethanol-induced latency to lose the righting reflex or sleep time. These findings indicate that genetic risk of enhanced ethanol intake at adolescence is still present at adulthood, long after the developmental window when the selective breeding occurred. Exposure to stress at adulthood triggers the vulnerability associated with this genetic risk, an effect associated with enhanced anxiety.

中文翻译：

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青春期期间对高和低乙醇摄入量的短期选择在压力诱导的乙醇饮酒中产生挥之不去的作用，并产生易焦虑的表型。

乙醇的使用在青少年中很普遍，但只有一些过渡到有问题的饮酒。重要的是要了解为什么青少年亚组的饮酒困难风险会有所不同。这项研究报告了一个短期选择计划，以产生Wistar大鼠品系（分别是高和低青春期饮酒，分别为ADHI和ADLO品系），这些产品在青春期饮​​酒方面存在显着差异。在成年期对ADHI和ADLO后代的S0世代和孝子代1（S1），S2和S3进行基础或应激诱导的乙醇摄入量的测试，或在多元同心方场测试中进行避难所寻求和冒险活动（ MSCF）。该研究产生的品系在青春期的自由选择乙醇饮用方面有显着差异。在成年期观察到选择的效果，超过进行选择的阶段：S1-ADHI成年雄性大鼠没有，但S1-ADLO成年雄性大鼠表现出应激诱导的饮酒。这些影响与寻求住房和冒险行为的重大改变有关。ADHI大鼠在需要进行冒险的MSCF区域花费的时间明显减少，而在黑暗的庇护区域花费的时间明显更多。通过施用0.5 g / kg乙醇可将其中一些影响归一化。乙醇引起的延迟恢复力或睡眠时间没有线性差异。这些发现表明，在成年期，发生选择性育种的发育窗口很久之后，仍然存在青春期增加乙醇摄入的遗传风险。成年后承受压力会触发与这种遗传风险相关的脆弱性，