Orthopedic surgical procedures based on the use of conventional biological graft tissues are often associated with serious post-operative complications such as immune rejection, bacterial infection, and poor osseointegration. Bioresorbable bone graft substitutes have emerged as attractive alternatives to conventional strategies because they can mimic the composition and mechanical properties of the native bone. Among these, bioactive glasses (BGs) hold great potential to be used as biomaterials for bone tissue engineering owing to their biomimetic composition and high biocompatibility and osteoinductivity. Here, we report the development of a novel composite biomaterial for bone tissue engineering based on the incorporation of a modified strontium- and lithium-doped 58S BG (i.e., BG-5/5) into gelatin methacryloyl (GelMA) hydrogels. We characterized the physicochemical properties of the BG formulation via different analytical techniques. Composite hydrogels were then prepared by directly adding BG-5/5 to the GelMA hydrogel precursor, followed by photocrosslinking of the polymeric network via visible light. We characterized the physical, mechanical, and adhesive properties of GelMA/BG-5/5 composites, as well as their in vitro cytocompatibility and osteoinductivity. In addition, we evaluated the antimicrobial properties of these composites in vitro, using a strain of methicillin-resistant Staphylococcus Aureus. GelMA/BG-5/5 composites combined the functional characteristics of the inorganic BG component, with the biocompatibility, biodegradability, and biomimetic composition of the hydrogel network. This novel biomaterial could be used for developing osteoinductive scaffolds or implant surface coatings with intrinsic antimicrobial properties and higher therapeutic efficacy.

中文翻译：

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具有抗菌性能的骨诱导可见光活化粘合复合材料的合成和表征。


基于使用传统生物移植组织的骨科手术通常会导致严重的术后并发症，例如免疫排斥、细菌感染和骨整合不良。生物可吸收骨移植替代品已成为传统策略的有吸引力的替代品，因为它们可以模仿天然骨的成分和机械性能。其中，生物活性玻璃（BG）由于其仿生成分、高生物相容性和骨诱导性，作为骨组织工程生物材料具有巨大的潜力。在这里，我们报告了一种用于骨组织工程的新型复合生物材料的开发，该材料基于将改性的锶和锂掺杂的 58S BG（即 BG-5/5）融入明胶甲基丙烯酰（GelMA）水凝胶中。我们通过不同的分析技术表征了 BG 制剂的理化性质。然后通过直接将 BG-5/5 添加到 GelMA 水凝胶前体中，然后通过可见光对聚合物网络进行光交联来制备复合水凝胶。我们表征了 GelMA/BG-5/5 复合材料的物理、机械和粘合性能，以及它们的体外细胞相容性和骨诱导性。此外，我们使用耐甲氧西林金黄色葡萄球菌菌株在体外评估了这些复合材料的抗菌特性。 GelMA/BG-5/5复合材料结合了无机BG组分的功能特性以及水凝胶网络的生物相容性、生物降解性和仿生组成。这种新型生物材料可用于开发具有内在抗菌特性和更高治疗功效的骨诱导支架或植入物表面涂层。