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Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2019-12-18 , DOI: 10.1021/acschembio.9b00842
Weiping Sun 1, 2, 3 , Yinbo Huo 1, 2, 3 , Yuxuan Mei 1 , Qingtong Zhou 4 , Suwen Zhao 1, 4 , Min Zhuang 1
Affiliation  

Proximity labeling has been used to study protein-protein interactions and can also be used as a protein labeling tool. We developed a novel 14-amino acid peptide substrate for the proximity-labeling enzyme PafA. The N terminus of the peptide can be modified with biotin or fluorophores, which allows various chemical moieties to be ligated to the target protein. We used PafA-mediated peptide labeling to label antibodies with a biotin tag without affecting the antigen binding capacity of the antibody. Similar strategies can be used for other types of protein labeling in the future.

中文翻译:

通过邻近标记识别可以与蛋白质结合的小探针。

邻近标记已用于研究蛋白质之间的相互作用,也可用作蛋白质标记工具。我们开发了一种新型14氨基酸肽底物,用于邻近标记酶PafA。可以用生物素或荧光团修饰肽的N末端,从而使各种化学部分与靶蛋白连接。我们使用PafA介导的肽标记来标记具有生物素标签的抗体,而不会影响抗体的抗原结合能力。将来,类似的策略也可以用于其他类型的蛋白质标记。
更新日期:2019-12-23
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