Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.,ACS Chemical Biology

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Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2019-12-18 , DOI: 10.1021/acschembio.9b00842
Weiping Sun _{1,

2,

3} , Yinbo Huo _{1,

2,

3} , Yuxuan Mei ₁ , Qingtong Zhou ₄ , Suwen Zhao _{1,

4} , Min Zhuang ₁

Affiliation

Proximity labeling has been used to study protein-protein interactions and can also be used as a protein labeling tool. We developed a novel 14-amino acid peptide substrate for the proximity-labeling enzyme PafA. The N terminus of the peptide can be modified with biotin or fluorophores, which allows various chemical moieties to be ligated to the target protein. We used PafA-mediated peptide labeling to label antibodies with a biotin tag without affecting the antigen binding capacity of the antibody. Similar strategies can be used for other types of protein labeling in the future.

中文翻译：

通过邻近标记识别可以与蛋白质结合的小探针。

邻近标记已用于研究蛋白质之间的相互作用，也可用作蛋白质标记工具。我们开发了一种新型14氨基酸肽底物，用于邻近标记酶PafA。可以用生物素或荧光团修饰肽的N末端，从而使各种化学部分与靶蛋白连接。我们使用PafA介导的肽标记来标记具有生物素标签的抗体，而不会影响抗体的抗原结合能力。将来，类似的策略也可以用于其他类型的蛋白质标记。

更新日期：2019-12-23

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