BACKGROUND Human periodontal ligament stem cells (hPDLSCs) are one of the most promising types of seed cells in periodontal tissue regeneration. Suitable biomaterials are additional essential components that must cooperate with seed cells for in vivo expansion or in vitro implantation. Extracellular matrix (ECM) derived from mesenchymal stem cells (MSCs) was recently reported to be a promising substrate with which to culture MSCs that could be applied in biomaterial scaffolds or bioink. Human urine-derived stem cells (hUSCs) have several advantages; their collection is non-invasive and easy, and hUSCs are low in cost, potentially making them a suitable and efficient source of ECM. The purpose of this study was to characterize the biological properties of ECM derived from hUSCs (UECM) and evaluate the effects of UECM on hPDLSCs. METHODS hPDLSCs grown on ECM derived from hPDLSCs (PECM) and fibronectin-coated tissue culture plastic (TCP) served as control groups. Both hUSCs and hPDLSCs were seeded on TCP and stimulated to produce ECM. After 8 days of stimulation, the samples were decellularized, leaving only ECM. Then, hPDLSCs were seeded onto UECM-, PECM-, and fibronectin-coated TCP and untreated TCP. RESULTS UECM consists of dense bundles of fibers which contain abundant fibronectin. Both UECM and PECM promoted hPDLSC proliferation, attachment, spreading, and differentiation. Between UECM and PECM, UECM enhanced proliferation, osteogenesis, and angiogenesis to a greater extent. Though fibronectin appeared to be the abundant component of UECM, its performance was inferior to that of UECM. CONCLUSIONS Our study provides an original perspective on different cell-specific ECMs and suggests UECM as a suitable biomaterial in which to culture hPDLSCs as UECM enhances their biological functions.

中文翻译：

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源自人尿来源的干细胞的细胞外基质增强了人牙周膜干细胞的扩增，粘附，扩散和分化。

背景技术人牙周膜干细胞（hPDLSC）是牙周组织再生中最有希望的种子细胞类型之一。合适的生物材料是必须与种子细胞协同作用以进行体内扩增或体外植入的其他必要成分。最近报道了源自间充质干细胞（MSC）的细胞外基质（ECM）是一种有前途的基质，可用于培养可用于生物材料支架或生物墨水的MSC。人尿来源的干细胞（hUSC）具有多个优点；它们的收集是非侵入性且容易的，并且hUSC成本低廉，可能使它们成为合适且有效的ECM来源。这项研究的目的是表征源自hUSCs（UECM）的ECM的生物学特性，并评估UECM对hPDLSCs的影响。方法将在hCMLSCs（PECM）和纤连蛋白包被的组织培养塑料（TCP）衍生的ECM上生长的hPDLSCs用作对照组。hUSC和hPDLSC均播种在TCP上，并刺激产生ECM。刺激8天后，将样品脱细胞，仅剩下ECM。然后，将hPDLSCs播种到UECM，PECM和纤连蛋白包被的TCP以及未处理的TCP上。结果UECM由密集的纤维束组成，其中包含丰富的纤连蛋白。UECM和PECM均促进hPDLSC增殖，附着，扩散和分化。在UECM和PECM之间，UECM在更大程度上增强了增殖，成骨和血管生成。尽管纤连蛋白似乎是UECM的丰富成分，但其​​性能不如UECM。