Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.

中文翻译：

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TRITEX：使用开源工具对小麦科基因组进行染色体规模的序列组装

染色体规模的基因组序列组装是泛基因组研究的基础。最近在大基因组小麦和大麦中进行的基因组组装工作依赖于商业闭源组装算法 DeNovoMagic。我们提出了 TRITEX，这是一种开源计算工作流程，它将双端、配对、10X 基因组学链接读取与染色体构象捕获测序数据相结合，以构建具有排序为染色体假分子的兆碱基级连续性的序列支架。我们评估了 TRITEX 在四倍体野生二粒小麦和六倍体面包小麦的公开序列数据上的性能，并构建了大麦品种 Morex 的改进注释参考基因组序列组装作为社区资源。