Analyzing the Substrate Specificity of a Class of Long-Horned-Beetle-Derived Xylanases by Using Synthetic Arabinoxylan Oligo- and Polysaccharides.,ChemBioChem

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Analyzing the Substrate Specificity of a Class of Long-Horned-Beetle-Derived Xylanases by Using Synthetic Arabinoxylan Oligo- and Polysaccharides.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-01-21 , DOI: 10.1002/cbic.201900687
Yannick Pauchet ₁ , Colin Ruprecht ₂ , Fabian Pfrengle _{2,

3}

Affiliation

Xylophagous long-horned beetles thrive in challenging environments. To access nutrients, they secrete plant-cell-wall-degrading enzymes in their gut fluid; among them are cellulases of the subfamily 2 of glycoside hydrolase family 5 (GH5_2). Recently, we discovered that several beetle-derived GH5_2s use xylan as a substrate instead of cellulose, which is unusual for this family of enzymes. Here, we analyze the substrate specificity of a GH5_2 xylanase from the beetle Apriona japonica (AJAGH5_2-1) using commercially available substrates and synthetic arabinoxylan oligo- and polysaccharides. We demonstrate that AJAGH5_2-1 processes arabinoxylan polysaccharides in a manner distinct from classical xylanase families such as GH10 and GH11. AJAGH5_2-1 is active on long oligosaccharides and cleaves at the non-reducing end of a substituted xylose residue (position +1) only if: 1) three xylose residues are present upstream and downstream of the cleavage site, and 2) xylose residues at positions -1, -2, +2 and +3 are not substituted.

中文翻译：

通过使用合成的阿拉伯木聚糖寡糖和多糖分析一类长角甲虫衍生的木聚糖酶的底物特异性。

木乃伊长角甲虫在充满挑战的环境中壮成长。为了获取营养，他们在肠液中分泌植物细胞壁降解酶。其中有糖苷水解酶家族5（GH5_2）的亚家族2的纤维素酶。最近，我们发现一些甲虫来源的GH5_2使用木聚糖代替纤维素作为底物，这对于该酶家族而言是不常见的。在这里，我们使用可商购的底物以及合成的阿拉伯木聚糖寡糖和多糖分析了甲虫Apriona japonica（AJAGH5_2-1）的GH5_2木聚糖酶的底物特异性。我们证明，AJAGH5_2-1以不同于经典木聚糖酶家族（例如GH10和GH11）的方式处理阿拉伯木聚糖多糖。

更新日期：2019-12-18

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