Design and application of single-cell RNA sequencing to study kidney immune cells in lupus nephritis.,Nature Reviews Nephrology

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Design and application of single-cell RNA sequencing to study kidney immune cells in lupus nephritis.
Nature Reviews Nephrology ( IF 28.6 ) Pub Date : 2019-12-18 , DOI: 10.1038/s41581-019-0232-6
Deepak A Rao ₁ , Arnon Arazi ₂ , David Wofsy ₃ , Betty Diamond ₄

Affiliation

The immune mechanisms that cause tissue injury in lupus nephritis have been challenging to define. The advent of high-dimensional cellular analyses, such as single-cell RNA sequencing, has enabled detailed characterization of the cell populations present in small biopsy samples of kidney tissue. In parallel, the development of methods that cryopreserve kidney biopsy specimens in a manner that preserves intact, viable cells, has enabled the uniform analysis of tissue samples collected at multiple sites and across many geographic areas and demographic cohorts with high-dimensional platforms. The application of these methods to kidney biopsy samples from patients with lupus nephritis has begun to define the phenotypes of both infiltrating and resident immune cells, as well as parenchymal cells, present in nephritic kidneys. The detection of similar immune cell populations in urine suggests that it might be possible to non-invasively monitor immune activation in kidneys. Once applied to large patient cohorts, these high-dimensional studies might enable patient stratification according to patterns of immune cell activation in the kidney or identify disease features that can be used as surrogate measures of efficacy in clinical trials. Applied broadly across multiple inflammatory kidney diseases, these studies promise to enormously expand our understanding of renal inflammation in the next decade.

中文翻译：

用于研究狼疮性肾炎中肾脏免疫细胞的单细胞RNA测序的设计和应用。

定义狼疮性肾炎中引起组织损伤的免疫机制具有挑战性。高维细胞分析（如单细胞RNA测序）的出现，使得能够对肾脏组织的小型活检样品中存在的细胞群体进行详细表征。同时，以保留完整的，有活力的细胞的方式冷冻保存肾脏活检标本的方法的发展，使得能够对在多个地点，跨多个地理区域和具有高维平台的人群进行收集的组织样本进行统一分析。这些方法在狼疮性肾炎患者的肾脏活检样本中的应用已经开始确定存在于肾肾脏中的浸润和驻留免疫细胞以及实质细胞的表型。在尿液中检测到相似的免疫细胞群表明，有可能非侵入性地监测肾脏的免疫激活。一旦应用于大型患者队列，这些高维度的研究就可以根据肾脏中免疫细胞激活的模式对患者进行分层，或者确定可以用作临床试验疗效替代指标的疾病特征。这些研究广泛应用于多种炎症性肾脏疾病，有望在未来十年极大地扩展我们对肾脏炎症的认识。这些高维度的研究可能会根据肾脏中免疫细胞激活的模式使患者分层，或者确定可以用作临床试验疗效替代指标的疾病特征。这些研究广泛应用于多种炎症性肾脏疾病，有望在未来十年极大地扩展我们对肾脏炎症的认识。这些高维度的研究可能会根据肾脏中免疫细胞激活的模式使患者分层，或者确定可以用作临床试验疗效替代指标的疾病特征。这些研究广泛应用于多种炎症性肾脏疾病，有望在未来十年极大地扩展我们对肾脏炎症的认识。

更新日期：2019-12-18

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