A near-infrared fluorescent probe reveals decreased mitochondrial polarity during mitophagy,Chemical Science

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A near-infrared fluorescent probe reveals decreased mitochondrial polarity during mitophagy
Chemical Science ( IF 7.6 ) Pub Date : 2019/12/18 , DOI: 10.1039/c9sc05505c
Xiaoyi Li _{1,

2,

3,

4,

5} , Xiaohua Li _{1,

2,

3,

4,

5} , Huimin Ma _{1,

2,

3,

4,

5}

Affiliation

Mitophagy is a selective form of autophagy by which dysfunctional and damaged mitochondria are degraded in autolysosomes. Since defective mitophagy is closely related to various pathological processes, investigation on the detailed mitophagy process is of great importance. In this respect, disclosing the alterations of mitochondrial microenvironments is expected to be a promising way. However, an appropriate method for monitoring the fluctuations of mitochondrial polarity during mitophagy is still lacking. Here, we report a near-infrared hydroxyl-hemicyanine fluorescent probe that responds to polarity exclusively. Both the shift of emission maxima and the fluorescence intensity ratios at two different wavelengths of the probe can be applied to quantifying the polarity accurately. With ratiometric fluorescence imaging, the polarity differences of normal and cancer cells are clearly discriminated. Most importantly, the mitochondrial polarity variations during starvation and drug-induced mitophagy are determined for the first time. The observed decrease of mitochondrial polarity during mitophagy, together with the rationally designed probe, may facilitate the study on the vital role of mitophagy in physiological and pathological bioprocesses.

中文翻译：

近红外荧光探针显示线粒体吞噬过程中线粒体极性降低

线粒体吞噬是自噬的一种选择性形式，通过这种方式，功能紊乱和受损的线粒体在自身溶酶体中降解。由于线粒体缺陷与各种病理过程密切相关，因此详细的线粒体过程研究非常重要。在这方面，揭示线粒体微环境的变化有望成为一种有前途的方法。但是，仍然缺少用于在线粒体吞噬过程中监测线粒体极性波动的适当方法。在这里，我们报告了一种仅对极性有反应的近红外羟基-半花菁荧光探针。探针的两个不同波长处的最大发射位移和荧光强度比都可用于精确定量极性。借助比例荧光成像，可以清楚地区分正常细胞和癌细胞的极性差异。最重要的是，首次确定了饥饿和药物诱导的线粒体吞噬过程中的线粒体极性变化。线粒体中线粒体极性的观察到的减少，以及合理设计的探针，可能有助于研究线粒体在生理和病理生物过程中的重要作用。

更新日期：2020-02-13

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