There presents a rapid development for fluorescent sensory systems in recent years, appended with multiple functions, including organelle targeting, near-infrared emission, photothermal property and so forth. These in-depth studies extend the application area undoubtedly, but an excellent responsive behavior still needs a precise control. This Review focuses on the chemical modulation for responsive performance, which is discussed mainly in two parts, improvement of signal-noise ratio and signal amplification strategy. In addition to multifaceted literatures to illustrate the ratiometric strategy, comments on background-free platform are summarized elaborately, which propose a total clearance in background by in situ reactions. As for proposal to modify the responsive spectra, the control on chemical equilibrium, introduction of an additional “amplifier” and self-assembly-featured spectra manipulated by analytes are elucidated in detail, aiming to provide a straightforward pathway to constitute a set of well-behaved receptors integrating analytes-sensing with dye-distribution, avoiding the increasing demands for a complex probe.

近年来，荧光传感系统迅速发展，并具有多种功能，包括细胞器靶向，近红外发射，光热特性等。这些深入的研究无疑扩大了应用范围，但是出色的响应行为仍然需要精确的控制。这篇综述的重点是响应性能的化学调制，主要分为两个部分：信噪比的改善和信号放大策略。除了用于说明比例策略的多方面文献外，还详细总结了对无背景平台的评论，提出了原位背景下的总清除率反应。至于修改响应光谱的建议，将详细说明化学平衡的控制，引入额外的“放大器”以及由分析物操纵的具有自组装特征的光谱，旨在为构成一组良好的色谱图提供直接途径。行为受体整合了分析物传感和染料分布，从而避免了对复杂探针的日益增长的需求。