Directional Immobilization of Proteins on Gold Nanoparticles Is Essential for Their Biological Activity: Leptin as a Case Study.,Bioconjugate Chemistry

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Directional Immobilization of Proteins on Gold Nanoparticles Is Essential for Their Biological Activity: Leptin as a Case Study.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-12-18 , DOI: 10.1021/acs.bioconjchem.9b00748
Vincenzo Mangini ₁ , Vito Maggi _{1,

2} , Alberta Trianni ₁ , Francesca Melle ₁ , Elisa De Luca ₁ , Antonio Pennetta _{2,

3} , Roberta Del Sole ₂ , Giovanni Ventura ₄ , Tommaso R I Cataldi _{4,

5} , Roberto Fiammengo ₁

Affiliation

Gold nanomaterials hold great potential for biomedical applications. While this field is evolving rapidly, little attention has been paid to precise nanoparticle design and functionalization. Here, we show that when using proteins as targeting moieties, it is fundamental to immobilize them directionally to preserve their biological activity. Using full-length leptin as a case study, we have developed two alternative conjugation strategies for protein immobilization based on either a site-selective or a nonselective derivatization approach. We show that only nanoparticles with leptin immobilized site-selectively fully retain the ability to interact with the cognate leptin receptor. These results demonstrate the importance of a specified molecular design when preparing nanoparticles labeled with proteins.

中文翻译：

蛋白质在金纳米颗粒上的定向固定对其生物活性至关重要：瘦素作为案例研究。

金纳米材料在生物医学应用中具有巨大的潜力。尽管该领域正在迅速发展，但很少有人关注精确的纳米颗粒设计和功能化。在这里，我们表明当使用蛋白质作为靶向部分时，至关重要的是将其定向固定以保持其生物学活性。使用全长瘦素作为案例研究，我们基于定点选择性或非选择性衍生化方法开发了两种替代的蛋白质固定偶联策略。我们显示，只有具有瘦素固定位点的纳米颗粒才能完全保留与同源瘦素受体相互作用的能力。这些结果证明了在制备用蛋白质标记的纳米颗粒时进行特定分子设计的重要性。

更新日期：2019-12-19

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