Conducting polymers tethered with molecular recognition elements are good candidates for biosensing applications such as detecting a target molecule with selectivity. We develop a new monomer, namely, 3,4-ethylenedioxythiophene bearing a pyridylboronic acid moiety (EDOT-PyBA), for label-free detection of sialic acid as a cancer biomarker. PyBA, which is known to show specific binding to sialic acid in acid conditions is used as a synthetic ligand instead of lectins. PyBA confirms the enhanced binding affinity for sialic acid at pH 5.0-6.0 compared with traditional phenylboronic acid. Poly(EDOT-PyBA) is electrodeposited on a planar glassy carbon electrode and the obtained film is successfully characterized by X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle measurements, and electrochemical impedance spectroscopy. The specific interaction of PyBA with sialic acid at the solution/electrode interface is detected by differential pulse voltammetry in a dynamic range 0.1-3.0 mM with a detection limit of 0.1 mM for a detection time of 3 min. The sensitivity covers the total level of free sialic acid in human serum and the assay time is the shorter than that of other methods. The poly(EDOT-PyBA) electrode successfully detects spiked sialic acid in human serum samples. Owing to its processability, mass productivity, and robustness, polythiophene conjugated with "boronolectin" is a candidate material for developing point-of-care and wearable biosensors.

中文翻译：

---

带有吡啶基硼酸基团的聚（3,4-乙撑二氧噻吩），可特异性识别唾液酸。

与分子识别元件束缚的导电聚合物是生物传感应用（例如选择性检测目标分子）的良好候选者。我们开发了一种新的单体，即带有吡啶基硼酸部分（EDOT-PyBA）的3,4-乙撑二氧噻吩，用于无标签检测唾液酸作为癌症的生物标志物。已知在酸性条件下会显示与唾液酸的特异性结合的PyBA被用作合成的配体，而不是凝集素。与传统的苯基硼酸相比，PyBA证实了在pH 5.0-6.0下对唾液酸的结合亲和力增强。将Poly（EDOT-PyBA）电沉积在平面玻碳电极上，并通过X射线光电子能谱，扫描电子显微镜，原子力显微镜，水接触角测量，和电化学阻抗谱。通过差分脉冲伏安法在0.1-3.0 mM的动态范围内以0.1 mM的检出限检测PyBA与唾液酸在溶液/电极界面的特异性相互作用，检测时间为3分钟。灵敏度涵盖了人血清中游离唾液酸的总含量，并且测定时间比其他方法短。聚（EDOT-PyBA）电极可成功检测人血清样品中的加标唾液酸。由于其可加工性，大规模生产性和耐用性，与“硼诺菌素”缀合的聚噻吩是用于开发即时医疗和可穿戴生物传感器的候选材料。通过差分脉冲伏安法在0.1-3.0 mM的动态范围内以0.1 mM的检出限检测PyBA与唾液酸在溶液/电极界面的特异性相互作用，检测时间为3分钟。灵敏度涵盖了人血清中游离唾液酸的总含量，并且测定时间比其他方法短。聚（EDOT-PyBA）电极可成功检测人血清样品中的加标唾液酸。由于其可加工性，大规模生产性和耐用性，与“硼诺菌素”缀合的聚噻吩是用于开发即时医疗和可穿戴生物传感器的候选材料。通过差分脉冲伏安法在0.1-3.0 mM的动态范围内以0.1 mM的检出限检测PyBA与唾液酸在溶液/电极界面的特异性相互作用，检测时间为3分钟。灵敏度涵盖了人血清中游离唾液酸的总含量，并且测定时间比其他方法短。聚（EDOT-PyBA）电极可成功检测人血清样品中的加标唾液酸。由于其可加工性，大规模生产性和耐用性，与“硼诺菌素”缀合的聚噻吩是用于开发即时医疗和可穿戴生物传感器的候选材料。灵敏度涵盖了人血清中游离唾液酸的总含量，并且测定时间比其他方法短。聚（EDOT-PyBA）电极可成功检测人血清样品中的加标唾液酸。由于其可加工性，大规模生产性和耐用性，与“硼诺菌素”缀合的聚噻吩是用于开发即时医疗和可穿戴生物传感器的候选材料。灵敏度涵盖了人血清中游离唾液酸的总含量，并且测定时间比其他方法短。聚（EDOT-PyBA）电极可成功检测人血清样品中的加标唾液酸。由于其可加工性，大规模生产性和耐用性，与“硼诺菌素”缀合的聚噻吩是用于开发即时医疗和可穿戴生物传感器的候选材料。