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Large Fecal Reservoir of Escherichia coli Sequence Type 131-H30 Subclone Strains That Are Shared Within Households and Resemble Clinical ST131-H30 Isolates.
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2020-04-27 , DOI: 10.1093/infdis/jiz669
Muhanad Mohamed 1 , Connie Clabots 2 , Stephen B Porter 2 , Tricia Bender 3 , Paul Thuras 4 , James R Johnson 1
Affiliation  

BACKGROUND Emerging antimicrobial-resistant Escherichia coli represent mainly the nested (fluoroquinolone-resistant [FQR]) H30R and H30Rx subclones within sequence type 131 (ST131). Intestinal colonization and within-household transmission may underlie H30R's emergence. METHODS We screened fecal samples from 741 volunteers (383 veterans, 358 household members, including pets) for ST131 and FQR E. coli (FQREC) and used molecular profiling to resolve unique strains. Selected strains underwent PCR-based detection of phylogroups, sequence types (STs), H30, H30Rx, and 53 virulence genes (VGs). Within-household strain sharing was compared with household, host, and bacterial characteristics. Fecal isolates were compared with clinical isolates. RESULTS Colonization prevalence was 5.1% for H30R, 8% for ST131 (67% FQREC), and 10% for FQREC (52% ST131). ST131 isolates exhibited more VGs than non-ST131 isolates. Strain sharing (27% of multisubject households, 18% of corresponding subjects) was associated with the elderly, FQREC, H30R, H30Rx, ST73, and specific VGs. Fecal ST131 and FQREC isolates resembled contemporaneous and historical clinical isolates according to all studied traits. CONCLUSIONS Veterans and their human household members commonly carry and extensively share FQREC, predominantly H30R, thereby likely facilitating the ST131 pandemic. Strain sharing corresponds with multiple bacterial characteristics, including FQ resistance and specific VGs, which may promote intestinal colonization and/or host-to-host transmission.

中文翻译:

大型粪便水库,在家庭内部共享且类似于临床ST131-H30分离株的序列类型131-H30亚克隆菌株。

背景技术新兴的抗药性大肠杆菌主要代表序列类型131(ST131)内的嵌套(抗氟喹诺酮[FQR])H30R和H30Rx亚克隆。肠道定植和家庭内部传播可能是H30R出现的基础。方法我们从741名志愿者(383名退伍军人,358名家庭成员,包括宠物)的粪便样本中筛选出了ST131和FQR E. coli(FQREC),并使用分子谱分析来解析独特的菌株。选定的菌株进行了基于PCR的系统群,序列类型(STs),H30,H30Rx和53种毒力基因(VGs)检测。将家庭内部菌株共享与家庭,宿主和细菌特征进行了比较。将粪便分离株与临床分离株进行了比较。结果H30R的定殖率为5.1%,ST131的定殖率为8%(FQREC为67%),FQREC的定殖率为10%(ST131为52%)。与非ST131分离株相比,ST131分离株表现出更多的VG。菌株共享(多主体家庭占27%,相应科目占18%)与老年人,FQREC,H30R,H30Rx,ST73和特定VG相关。根据所有研究的特征,粪便ST131和FQREC分离株与同期和历史临床分离株相似。结论退伍军人及其家庭成员通常携带并广泛共享FQREC(主要是H30R),从而可能促进ST131大流行。菌株共享与多种细菌特征相对应,包括FQ抗性和特定的VG,它们可能会促进肠道定植和/或宿主之间的传播。18%的相应受试者与老年人,FQREC,H30R,H30Rx,ST73和特定VG相关。根据所有研究的特征,粪便ST131和FQREC分离株与同期和历史临床分离株相似。结论退伍军人及其家庭成员通常携带并广泛共享FQREC(主要是H30R),从而可能促进ST131大流行。菌株共享与多种细菌特征相对应,包括FQ抗性和特定的VG,它们可能会促进肠道定植和/或宿主之间的传播。18%的相应受试者)与老年人,FQREC,H30R,H30Rx,ST73和特定VG相关。根据所有研究的特征,粪便ST131和FQREC分离株与同期和历史临床分离株相似。结论退伍军人及其家庭成员通常携带并广泛共享FQREC(主要是H30R),从而可能促进ST131大流行。菌株共享与多种细菌特征相对应,包括FQ抗性和特定的VG,它们可能会促进肠道定植和/或宿主之间的传播。主要是H30R,从而可能促进ST131大流行。菌株共享与多种细菌特征相对应,包括FQ抗性和特定的VG,它们可能会促进肠道定植和/或宿主之间的传播。主要是H30R,从而可能促进ST131大流行。菌株共享与多种细菌特征相对应,包括FQ抗性和特定的VG,它们可能会促进肠道定植和/或宿主之间的传播。
更新日期:2019-12-19
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