Synchronous 3D Bioprinting of Large-Scale Cell-Laden Constructs with Nutrient Networks.,Advanced Healthcare Materials

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Synchronous 3D Bioprinting of Large-Scale Cell-Laden Constructs with Nutrient Networks.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2019-12-17 , DOI: 10.1002/adhm.201901142
Lei Shao ₁ , Qing Gao ₁ , Chaoqi Xie ₁ , Jianzhong Fu ₁ , Meixiang Xiang ₂ , Yong He _{1,

3}

Affiliation

Maintaining an adequate supply of nutrients/oxygen is a major challenge in the biofabrication of large tissue constructs. However, building preformed nutrient networks may be an effective strategy for engineering thick tissues. Here, a novel way for bioprinting large‐scale tissue constructs with intentional nutrient networks is presented. A special nozzle is developed which can print bioink and sacrificial ink half and half synchronously in a single filament. Nutrient networks of these bioprinted constructs are formed by subsequently dissolving away gelatin, which allows for effective oxygen, nutrient, and waste diffusion, facilitating the cell activity and the generation of functional tissues. Due to the cell‐laden bioink and sacrificial ink working together and promoting each other's printability to support themselves, complex soft cell‐laden constructs with nutrient networks can easily be printed. Furthermore, two different cell types (osteoblast, human umbilical vein endothelial cells) encapsulated in the bioprinted large‐scale constructs (≥1 cm) with nutrient networks show enhanced cell viability and spreading within a period of culture. It is envisioned that the advanced bioprinting technology may have significant potentials in facilitating the engineering of complex structures for tissue‐specific needs, and bioprinting large‐scale tissue constructs with nutrient networks toward applications in organ transplantation and repair.

中文翻译：

带有营养网络的大型细胞构建体的同步3D生物打印。

维持足够的营养/氧气供应是大型组织构造物生物制造中的主要挑战。但是，建立预先建立的营养网络可能是对厚组织进行工程改造的有效策略。在这里，提出了一种新的方法，用有意的营养网络对大型组织结构进行生物打印。开发了一种特殊的喷嘴，它可以在一根细丝中同步打印一半和一半的生物墨水和牺牲性墨水。这些生物印刷结构的营养网络是通过随后溶解掉明胶形成的，明胶允许有效的氧气，养分和废物扩散，从而促进细胞活性和功能组织的生成。由于充满细胞的生物墨水和牺牲墨水可以协同工作，并促进彼此的可打印性以维持自身，带有营养网络的复杂的充满软细胞的结构可以轻松打印。此外，封装在具有营养网络的生物打印大规模构建体（≥1 cm）中的两种不同细胞类型（成骨细胞，人脐静脉内皮细胞）显示出增强的细胞活力并在培养期内扩散。可以预见，先进的生物打印技术在促进复杂结构满足组织特定需求的工程以及利用营养网络对大型组织构建物进行生物打印以在器官移植和修复中的应用方面具有巨大潜力。带有营养网络的生物打印的大型构建体（≥1 cm）中封装的人脐静脉内皮细胞显示出增强的细胞活力并在培养期内扩散。可以预见，先进的生物打印技术在促进复杂结构满足组织特定需求的工程以及利用营养网络对大型组织构建物进行生物打印以在器官移植和修复中的应用方面具有巨大潜力。带有营养网络的生物打印的大型构建体（≥1 cm）中封装的人脐静脉内皮细胞显示出增强的细胞活力并在培养期内扩散。可以预见，先进的生物打印技术在促进复杂结构满足组织特定需求的工程以及利用营养网络对大型组织构建物进行生物打印以在器官移植和修复中的应用方面具有巨大潜力。

更新日期：2019-12-17

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