The mechanisms regulating, and modulating potato wound-healing processes are of great importance in reducing tuber infections, reducing shrinkage and maintaining quality and nutritional value for growers and consumers. Wound-induced changes in tuber polyamine metabolism have been linked to the modulation of wound healing (WH) and in possibly providing the crucial amount of H2O2 required for suberization processes. In this investigation we determined the effect of inhibition of specific steps within the pathway of polyamine metabolism on polyamine content and the initial accumulation of suberin polyphenolics (SPP) during WH. The accumulation of SPP represents a critical part of the beginning or inchoate phase of tuber WH during closing-layer formation because it serves as a barrier to bacterial infection and is a requisite for the accumulation of suberin polyaliphatics which provide the barrier to fungal infection. Results showed that the inhibitor treatments that caused changes in polyamine content generally did not influence wound-induced accumulation of SPP. Such lack of correlation was found for inhibitors involved in metabolism and oxidation of putrescine (arginine decarboxylase, ornithine decarboxylase, and diamine oxidase). However, accumulation of SPP was dramatically reduced by treatment with guazatine, a potent inhibitor of polyamine oxidase (PAO), and methylglyoxal-bis(guanylhydrazone), a putative inhibitor of S-adenosylmethione decarboxylase which may also cross-react to inhibit PAO. The mode of action of these inhibitors is presumed to be blockage of essential H2O2 production within the WH cell wall. These results are of great importance in understanding the mechanisms modulating WH and ultimately controlling related infections and associated postharvest losses.

中文翻译：

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三聚胺和四聚胺氧化的抑制剂会损害伤口引起的木栓化的初始阶段，但不会损害二胺氧化


调节马铃薯伤口愈合过程的机制对于减少块茎感染、减少收缩以及保持种植者和消费者的质量和营养价值非常重要。伤口引起的块茎多胺代谢变化与伤口愈合 (WH) 的调节有关，并可能提供木栓化过程所需的关键量的 H2O2。在这项研究中，我们确定了多胺代谢途径中特定步骤的抑制对多胺含量和 WH 期间木栓质多酚 (SPP) 初始积累的影响。 SPP 的积累代表了块茎 WH 在闭合层形成过程中的开始或早期阶段的关键部分，因为它充当细菌感染的屏障，并且是木栓质多脂族物质积累的必要条件，木栓质多脂族物质提供了真菌感染的屏障。结果表明，引起多胺含量变化的抑制剂处理通常不会影响伤口诱导的 SPP 积累。对于参与腐胺代谢和氧化的抑制剂（精氨酸脱羧酶、鸟氨酸脱羧酶和二胺氧化酶），发现这种相关性的缺乏。然而，通过使用瓜扎汀（多胺氧化酶（PAO）的有效抑制剂）和甲基乙二醛-双（脒基腙）（一种假定的S-腺苷甲硫酮脱羧酶抑制剂，也可能发生交叉反应来抑制PAO）处理，SPP的积累显着减少。这些抑制剂的作用方式被认为是阻断 WH 细胞壁内必需的 H2O2 产生。 这些结果对于理解 WH 调节机制并最终控制相关感染和相关采后损失具有重要意义。