Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λem 427 nm (λex 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X-100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06-1.0 μg ml-1 with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml-1 , respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components.

中文翻译：

---

微环境改进方案，用于灵敏的荧光分光光度法测定丙型肝炎病毒抗病毒药物（Simeprevir）：应用于人血浆。

Simeprevir（SPV）是一种功能强大的抗丙型肝炎病毒药物，最近被引入制药市场。我们在这里建立并验证了一种简便，简单且灵敏的荧光光谱法，用于在λem427 nm（λex337 nm）处进行估计。建议的程序基于通过添加Triton X-100改变SPV在缓冲水溶液中的微环境，从而将SPV的原始排放提高了两倍。SPV浓度与观察到的荧光强度之间的关系在0.06-1.0μgml-1范围内呈线性关系，相关系数为0.9997。检测限和定量限分别为21和64 ng ml-1。