Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis.,ChemBioChem

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Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-01-20 , DOI: 10.1002/cbic.201900675
Jack G Ganley ₁ , Hannah K D'Ambrosio ₁ , Meg Shieh ₁ , Emily R Derbyshire _{1,

2}

Affiliation

Anopheles mosquito microbiomes are intriguing ecological niches. Within the gut, microbes adapt to oxidative stress due to heme and iron after blood meals. Although metagenomic sequencing has illuminated spatial and temporal fluxes of microbiome populations, limited data exist on microbial growth dynamics. Here, we analyze growth interactions between a dominant microbiome species, Elizabethkingia anophelis, and other Anopheles-associated bacteria. We find E. anophelis inhibits a Pseudomonas sp. via an antimicrobial-independent mechanism and observe biliverdins, heme degradation products, upregulated in cocultures. Purification and characterization of E. anophelis HemS demonstrates heme degradation, and we observe hemS expression is upregulated when cocultured with Pseudomonas sp. This study reveals a competitive microbial interaction between mosquito-associated bacteria and characterizes the stimulation of heme degradation in E. anophelis when grown with Pseudomonas sp.

中文翻译：

蚊子-微生物组细菌的共培养可促进伊蚊伊丽莎白女王的血红素降解。

按蚊蚊子微生物群是令人着迷的生态位。在肠道内，餐后微生物适应血红素和铁引起的氧化应激。尽管宏基因组测序已阐明了微生物组种群的时空通量，但有关微生物生长动力学的数据有限。在这里，我们分析了主要的微生物组物种伊丽莎白女王按蚊和其他按蚊相关细菌之间的生长相互作用。我们发现按蚊大肠杆菌抑制了假单胞菌。通过不依赖抗生素的机制，观察共培养物中上调的胆绿素，血红素降解产物。大肠杆菌的HemS的纯化和鉴定表明血红素降解，我们观察到与假单胞菌sp共培养时hemS的表达上调。

更新日期：2020-01-20

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