Short chain fructo-oligosaccharides (SC-FOS) are the potential prebiotics possessing diverse applications in both food and feed industries. The present study was aimed to extract inulin from chicory roots followed by its conversion into SC-FOS applying endoinulinase from Aspergillus fumigatus. The inulin was extracted from chicory roots through boiling in hot water, followed by precipitation with ethanol at room temperature or freezing condition. Maximum yield (42%) of inulin was obtained with three volumes of chilled absolute ethanol at room temperature. HPLC analysis of enzymatic hydrolysate detected kestose (GF2), nystose (GF3), and other FOS having higher degree of polymerization (DP). Maximum GF2 (5.79 mg/ml) was detected at temperature 50 °C, pH 5.5 with 2 U of enzyme dose after 6 h of hydrolysis; while maximum GF3 (4.33 mg/ml) was recorded at 60 °C, 5.5 pH with 0.5 U enzyme dose after 2 h of hydrolysis. Nevertheless, complete hydrolysis of inulin was noticed with 99% total oligosaccharide yield at 55 °C, 5.5 pH with 0.5 U enzyme dose after 4 h of hydrolysis with negligible amount of mono- and di-saccharides. The present finding demonstrated the process for higher yield of inulin from chicory roots followed by its conversion into SC-FOS applying fungal endoinulinase.

中文翻译：

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利用真菌内切菊粉酶从菊苣根菊粉中生产短链低聚果糖。


短链低聚果糖（SC-FOS）是潜在的益生元，在食品和饲料行业具有多种应用。本研究旨在从菊苣根中提取菊粉，然后利用烟曲霉内切菊粉酶将其转化为 SC-FOS。通过在热水中煮沸，然后在室温或冷冻条件下用乙醇沉淀，从菊苣根中提取菊粉。在室温下使用三体积的冷冻无水乙醇获得菊粉的最大产率（42%）。酶水解产物的 HPLC 分析检测到 kestose (GF2)、nystose (GF3) 和其他具有较高聚合度 (DP) 的 FOS。水解 6 小时后，在温度 50 °C、pH 5.5、2 U 酶剂量下检测到最大 GF2（5.79 mg/ml）；水解 2 小时后，在 60 °C、5.5 pH、0.5 U 酶剂量下记录到最大 GF3（4.33 mg/ml）。然而，在 55 °C、5.5 pH、0.5 U 酶剂量下，水解 4 小时后，菊粉完全水解，总寡糖产率为 99%，单糖和二糖的量可忽略不计。目前的发现证明了从菊苣根中获得更高菊粉产量，然后利用真菌内菊粉酶将其转化为 SC-FOS 的过程。