This paper presented a facile and efficient HPTLC method for quantifying riboflavin fortification in rice noodle that was a staple food popular in Asian countries. After high-throughput separation on silica gel plates with methanol/ethyl acetate/triethylamine (3/7/1, v/v/v) as the mobile phase, the obtained results were analyzed by fluorescence densitometry (mercury lamp, 360 nm excitation wavelength in combination with K400 optical filter). Quantification in this way offered high linearity (R² = 0.9998 within 10–80 ng/band) and precision (RSD < 5.2%), adequate detectability (LOD = 0.1 and LOQ = 0.3 mg/kg), and accuracy (spike-recovery rates within 91.4–105.1%, RSD < 8.1%). While yielding good comparison to conventional HPLC detection, the developed quantification featured superiorly high efficiency. Apart from that, the separation results were further evaluated by SERS. By jointly using sodium borohydride reduced AgNPs as the active substrate and 633 nm incident laser wavelength, the developed HPTLC-SERS detection gave clear and sensitive fingerprint-like proof which enabled unambiguous confirmation by visual inspection. Balancing well between simplicity, detectability, efficiency, and reliability, the proposed method exemplarily shown the promising applicability of HPTLC as a versatile tool in food analysis.

中文翻译：

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HPTLC密度测定法测定米粉中强化的核黄素含量：通过SERS指纹图谱确认

本文提出了一种简便而高效的HPTLC方法，用于定量米粉中的核黄素强化，米粉是亚洲国家的主要食品。在以甲醇/乙酸乙酯/三乙胺（3 /7/1 ，v / v / v）为流动相的硅胶板上高通量分离后，通过荧光光度法（汞灯，360 nm激发波长）分析获得的结果与K400光学滤镜结合使用）。以这种方式进行定量可提供高线性度（R ² 在10–80 ng / band内= 0.9998）和精度（RSD <5.2％），足够的可检测性（LOD = 0.1和LOQ = 0.3 mg / kg）和精度（加标回收率在91.4–105.1％之内，RSD <8.1 ％）。与传统的HPLC检测方法相比，该方法的定量结果具有很高的效率。除此之外，分离结果还通过SERS进行了评估。通过联合使用硼氢化钠还原的AgNPs作为活性底物和633 nm入射激光波长，开发的HPTLC-SERS检测可提供清晰灵敏的指纹样证据，从而可以通过目视检查进行明确确认。在简单性，可检测性，效率和可靠性之间取得了很好的平衡，该方法示例性地显示了HPTLC作为食品分析中的一种多功能工具的应用前景。