Microorganisms cope with a wide range of environmental challenges using different mechanisms. Their ability to prosper at extreme ambient pH and high temperatures has been well reported, but the adaptation mechanism often remains unrevealed. In this study, we addressed the dynamics of lipid and sugar profiles upon different cultivation conditions. The results showed that the cells grown at various pH and optimal temperature contained mannitol as the major cytosol sugar alcohol. The elevated temperature of 38 °C led to a two- to three-fold increase in total cytosol sugars with concurrent substitution of mannitol for trehalose. Lipid composition in the cells at optimal temperature changed insignificantly at any pH tested. The increase in the temperature caused some drop in the storage and membrane lipid levels, remarkable changes in their composition, and the degree of unsaturated fatty acids. It was shown that the fatty acid composition of some membrane phospholipids varied considerably at changing pH and temperature values. The data showed a pivotal role and flexibility of the sugar and lipid composition of Y. lipolytica W29 in adaptation to unfavorable environmental conditions.

中文翻译：

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在不同温度和pH下解脂耶氏酵母酵母中的可溶性糖和脂质调节。

微生物使用不同的机制来应对各种各样的环境挑战。已经报道了它们在极端的环境pH值和高温下能够繁荣发展的能力，但其适应机制通常仍未揭示。在这项研究中，我们研究了不同培养条件下脂质和糖分布的动态变化。结果表明，在不同pH和最适温度下生长的细胞均含有甘露醇作为主要的胞质溶胶醇。38°C的高温导致总胞浆糖增加了2到3倍，同时甘露醇替代了海藻糖。在任何测试的pH值下，最佳温度下细胞中的脂质组成变化都很小。温度升高导致储存和膜脂水平下降，它们的组成以及不饱和脂肪酸的程度发生了显着变化。结果表明，某些膜磷脂的脂肪酸组成随pH和温度值的变化而变化很大。数据显示解脂耶氏酵母W29的糖和脂质成分在适应不利环境条件方面的关键作用和灵活性。