Electrochemiluminescence detection of cardiac troponin I based on Au-Ag alloy nanourchins.,Analyst

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Electrochemiluminescence detection of cardiac troponin I based on Au-Ag alloy nanourchins.
Analyst ( IF 3.6 ) Pub Date : 2019-12-17 , DOI: 10.1039/c9an01904a
Minghan Wang ₁ , Junjie Liu , Xiaoli Qin , Xin Nie , Yifan Dong , Xu Liang , Zhiwei Zhu , Di Yang , Yuanhua Shao

Affiliation

In this work, an Au-Ag alloy nanourchin (Au-Ag alloy NU) based electrochemiluminescent (ECL) sensor for the measurement of cardiac troponin I (cTnI) was developed. The as-prepared Au-Ag alloy NUs exhibited higher specific surface area and better conductivity owing to their unique urchin-like morphology, which resulted in excellent electrocatalytic activity towards H2O2 in the luminol-H2O2 ECL system. We have found that the Au-Ag alloy NUs could enhance the ECL signal in the luminol-H2O2 solution. Based on these facts, a facile and label-free ECL immunosensor has been constructed for the analysis of cTnI, a cardiac biomarker, with a wide linear range of 3.5 pg mL-1-350 μg mL-1. This novel ECL immunosensor has good stability and reproducibility, showing potential application in clinical diagnostics. In addition, a non-enzymatic electrochemical sensor for H2O2 was also fabricated, with a wide linear detection range of 100 nM-200 μM, a low limit of detection of 45 nM and a fast response time (less than 2 s).

中文翻译：

基于Au-Ag合金纳米顽皮的心脏肌钙蛋白I的电化学发光检测。

在这项工作中，开发了一种用于测量心肌肌钙蛋白I（cTnI）的基于Au-Ag合金的纳米顽童（Au-Ag合金NU）的电化学发光（ECL）传感器。所制备的Au-Ag合金NUs由于具有独特的类似顽童的形态而具有更高的比表面积和更好的电导率，从而在luminol-H2O2 ECL系统中对H2O2具有出色的电催化活性。我们发现Au-Ag合金NUs可以增强鲁米诺-H2O2溶液中的ECL信号。基于这些事实，已构建了一种简便且无标记的ECL免疫传感器，用于分析cTnI（一种心脏生物标记物），线性范围为3.5 pg mL-1-350μgmL-1。这种新型的ECL免疫传感器具有良好的稳定性和可重复性，显示出在临床诊断中的潜在应用。此外，

更新日期：2020-02-13

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