Development of an on-line immobilized α-glucosidase microreactor coupled to liquid chromatography for screening of α-glucosidase inhibitors.,Journal of Pharmaceutical and Biomedical Analysis

当前位置： X-MOL 学术 › J. Pharmaceut. Biomed. Anal. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of an on-line immobilized α-glucosidase microreactor coupled to liquid chromatography for screening of α-glucosidase inhibitors.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2019-12-17 , DOI: 10.1016/j.jpba.2019.113047
Beibei Qiu ₁ , Yuqiong Shi ₁ , Luyun Yan ₁ , Xiangrong Wu ₁ , Jinhua Zhu ₁ , Dongbao Zhao ₂ , Md Zaved H Khan ₃ , Xiuhua Liu ₁

Affiliation

In order to rapidly screen α-glucosidase (α-GLU) inhibitors from Chinese herbs extract, an online screening method was developed by using enzymatic microreactors in combination with HPLC. A type of dodecahedral and porous material (ZIF-90) was synthesized at room temperature and employed as supports to construct enzymatic microreactor. The amount of α-glucosidase immobilized on ZIF-90 was 58.65 μg per mg carrier under the optimized conditions. In the online screening process, the eluent of 30 s was selected for detection. For the application of this on-line screening system, three α-glucosidase inhibitors with known structure (2,4-dimethoxy-6,7-dihydroxyphenanthrene, batatasin I, 3,5-dimethoxy-2'-hydroxyaiaryl) were selectively extracted from Dioscorea opposita Thunb. Three compounds screened from honeysuckle leaves were isochlorogenic acid B, 1,5-dicaffeoylquinic acid and isochlorogenic acid C, respectively. Two compounds including (+)-catechin and (-)-epigallocatechin gallate were screened from Xinyang Maojian tea. Three unknown ingredients were also screened out from Radix Rehmanniae Praeparata. The nanomaterials in the microreactor can be conveniently replaced. The screening flow rate and elution time can be easily adjusted and controlled by microinjection pump. Considering the specificity of enzyme binding and convenience of online screening system, this method has great potential for fast real-time fishing of α-glucosidase inhibitors from Chinese herbal medicines.

中文翻译：

在线固定化α-葡萄糖苷酶微反应器与液相色谱联用的筛选，用于筛选α-葡萄糖苷酶抑制剂。

为了从中草药提取物中快速筛选α-葡萄糖苷酶（α-GLU）抑制剂，通过使用酶促微反应器结合HPLC建立了在线筛选方法。在室温下合成了一种十二面体和多孔材料（ZIF-90），并用作载体来构建酶促微反应器。在最佳条件下，固定在ZIF-90上的α-葡萄糖苷酶的量为58.65μg/ mg载体。在在线筛选过程中，选择了30 s的洗脱液进行检测。为了应用该在线筛选系统，从中选择性地提取了三种具有已知结构的α-葡萄糖苷酶抑制剂（2,4-二甲氧基-6,7-二羟基菲，batatasin I，3,5-二甲氧基-2'-羟基芳基）薯对生。从金银花叶片中筛选出的三种化合物是异绿原酸B，1,5-二咖啡酰奎尼酸和异绿原酸C。从信阳毛尖茶中筛选了两种化合物，包括（+）-儿茶素和（-）-表没食子儿茶素没食子酸酯。还从地黄中筛选出三种未知成分。微反应器中的纳米材料可以方便地更换。筛选流速和洗脱时间可通过微注射泵轻松调节和控制。考虑到酶结合的特异性和在线筛选系统的便利性，该方法具有快速实时捕捞中草药α-葡萄糖苷酶抑制剂的潜力。还从地黄中筛选出三种未知成分。微反应器中的纳米材料可以方便地更换。筛选流速和洗脱时间可以通过微量注射泵轻松调节和控制。考虑到酶结合的特异性和在线筛选系统的便利性，该方法具有快速实时捕捞中草药α-葡萄糖苷酶抑制剂的潜力。还从地黄中筛选出三种未知成分。微反应器中的纳米材料可以方便地更换。筛选流速和洗脱时间可以通过微量注射泵轻松调节和控制。考虑到酶结合的特异性和在线筛选系统的便利性，该方法具有快速实时捕捞中草药α-葡萄糖苷酶抑制剂的潜力。

更新日期：2019-12-18

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11