In order to evaluate dermal absorption during typical working conditions in family farming, the amount of dimethoate on clothing and in the stratum corneum (SC) was measured in three rural workers. This was achieved by using cotton patches on the worker’s clothes and SC quantification by the tape stripping approach. To mimic the above study, an in vitro approach was performed using Franz cells by applying dimethoate (0.4 and 1.8 μg) direct to pig skin or, on a section of cotton before contact with pig skin. The in vivo case results demonstrated the high levels and variability of agrochemicals to which these farmers are subjected, with the total potential dermal absorption between 0.87–2.85 mg/person/h and the estimated SC penetration factor (PF) between 0–54.0 and 0–28.9 % for the back of the neck and the arms respectively. This probably demonstrates the impact of correct protective clothing. For the in vitro study, the amount of pesticide retained in the SC was 52.63 ± 10.73and 135.15 ± 31.8 ng/cm² after applying 0.4 and 1.8 μg of pesticide directly on SC, and demonstrated close agreement with the in vivo approach. Further studies performed with this and other pesticides with different characteristics will contribute to the understanding of their transport through the skin.

为了评估家庭农业中典型工作条件下的真皮吸收情况，对三名农村工人的衣服和角质层（SC）中的乐果含量进行了测量。这是通过在工人的衣服上使用棉布补丁并通过胶带剥离法进行SC量化来实现的。为了模拟上述研究，使用Franz细胞通过在猪皮肤上或在接触猪皮肤的一部分棉花上直接施用乐果（0.4和1.8μg）进行体外方法。在体内病例结果表明，这些农户所使用的农用化学品水平高且易变，总潜在皮肤吸收量在0.87至2.85 mg /人/小时之间，估计的SC渗透因子（PF）在0至54.0至0至28.9％之间。分别用于脖子的后部和手臂。这可能说明正确的防护服的影响。对于体外研究，农药的量保留在SC为52.63±10.73and 135.15±31.8毫微克/厘米²直接在SC施用0.4和1.8微克的农药后，并表现出紧密的协议体内方法。用这种农药和其他具有不同特性的农药进行的进一步研究将有助于理解它们在皮肤中的运输。