Nanotechnology is an emerged field to develop the plant mediated metal based nanodrugs by green method. In this current study, the zinc oxide metal based nanoparticles were developed using (Clausena lansium (Lour.) Skeels) Peel aqueous extracts and zinc nitrate. The C.L extract zinc nanoparticleswere indicated by the sharp peak seen at 350 nm utilizing the Ultraviolet-Visible spectroscopy (UV-Vis). The high peaks indicate the presence of phytochemicals and its functional groups in ZnONPs were studied by the Fourier Transform Infrared Spectroscopy (FT-IR). The X-Ray Diffraction analysis (XRD) explores the pattern and structure of ZnONPs as spherical and base-centered monoclinic crystalline shapes. The C.L extract with Zn nanoparticles were spherical in nature and the size of the synthesized particles were about 28.42 nm respectively. The autophagy (Beclin-1, LC3-I, LC3-II and ATG4B) and apoptotic (Bax, Bcl-2 and Caspase-3) proteins were regulated by the treatment with ZnONPs in SH-SY5Y neuroblastoma cells. The DNA loss or damage was occurred in the ZnONPs treatment and it was performed using Comet assay. The ZnONPs treatment generates the ROS in the cells and decreased its stability and viability. Addition of NAC prevents ROS in the cultured SH-SY5Y cells and prevents the cells from the apoptosis. We concluded that the ZnONPs potentially kills the neuroblastoma cells by producing the intracellular ROS.

中文翻译：

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（Clausena lansium（Lour。）Skeels）果皮ZnONPs通过调节自噬机制对神经母细胞瘤（SH-SY5Y）细胞的抗癌和遗传毒性作用。

纳米技术是通过绿色方法开发植物介导的金属基纳米药物的新兴领域。在本研究中，使用（Clausena lansium（Lour。）Skeels）果皮水提取物和硝酸锌开发了基于氧化锌金属的纳米粒子。利用紫外线-可见光谱法（UV-Vis）在350nm处看到的尖峰表明了CL提取物锌纳米颗粒。高峰表明通过傅立叶变换红外光谱（FT-IR）研究了ZnONP中的植物化学成分及其官能团。X射线衍射分析（XRD）探索了ZnONP的球形和以碱为中心的单斜晶体形状的模式和结构。具有锌纳米颗粒的CL提取物本质上是球形的，并且合成的颗粒的尺寸分别为约28.42nm。自噬（Beclin-1，LC3-I，LC3-II和ATG4B）和凋亡蛋白（Bax，Bcl-2和Caspase-3）通过ZnONPs处理对SH-SY5Y神经母细胞瘤细胞进行调节。ZnONPs处理中发生了DNA丢失或损坏，并使用Comet分析进行。ZnONPs处理会在细胞中产生ROS，并降低其稳定性和生存能力。加入NAC可防止培养的SH-SY5Y细胞中的ROS并防止细胞凋亡。我们得出的结论是，ZnONPs可能通过产生细胞内ROS杀死神经母细胞瘤细胞。ZnONPs处理会在细胞中产生ROS，并降低其稳定性和生存能力。加入NAC可防止培养的SH-SY5Y细胞中的ROS并防止细胞凋亡。我们得出的结论是，ZnONPs可能通过产生细胞内ROS杀死神经母细胞瘤细胞。ZnONPs处理会在细胞中产生ROS，并降低其稳定性和生存能力。加入NAC可防止培养的SH-SY5Y细胞中的ROS并防止细胞凋亡。我们得出的结论是，ZnONPs可能通过产生细胞内ROS杀死神经母细胞瘤细胞。