Dengue non-structural protein 1 (NS1 DENV) is considered a biomarker for dengue fever in an early stage. A sensitive and rapid assay for distinguishing positive from negative dengue infection samples is imperative for epidemic control and public health in tropical regions because it enables the development of instantaneous updatable databases and effective surveillance systems. Presently, we successfully report, for the first time, the use of the electrochemical capacitive method for the detection of NS1 DENV biomarker in human serum samples. By using a ferrocene-tagged peptide modified surface containing anti-NS1 as the receptor, it was possible to differentiate positive from negative samples with a p < 0.01 in a reagentless and label-free capacitive format. This capacitive assay had a cut-off of 1.36% (confidence interval of 99.99%); it therefore opens new avenues for developing miniature label-free electrochemical devices for infectious diseases.

中文翻译：

---

登革热病毒感染的血清学现场护理和无标记电容性诊断。

登革热非结构蛋白1（NS1 DENV）在早期被认为是登革热的生物标志物。对于热带地区的流行病控制和公共卫生，必须有灵敏，快速的方法来区分阳性和阴性登革热感染样本，因为它可以开发即时可更新的数据库和有效的监视系统。目前，我们首次成功报告了使用电化学电容法检测人血清样品中NS1 DENV生物标志物的方法。通过使用含有抗NS1作为受体的二茂铁标记的肽修饰的表面，可以以无试剂和无标记的电容形式将ap <0.01的阳性样品与阴性样品区分开。该电容测定的截止值为1.36％（置信区间为99.99％）；