Here we report a methionine sulfoxide reductase B (MsrB) enzymatic system for the preparation of (S)-sulfoxides through kinetic resolution of racemic (rac) sulfoxides. Eight MsrB homologue recombinant proteins were expressed and their activities on asymmetric reduction of rac-sulfoxides were analyzed. Among these MsrB homologue proteins, one protein from Acidovorax species showed good activity and enantioselectivity towards several aryl-alkyl sulfoxides. The (S)-sulfoxides were prepared with 93–98% enantiomeric excess through kinetic resolution at initial substrate concentration up to 50 mM. The establishment of MsrB catalytic kinetic resolution system provides a new efficient green strategy for preparation of (S)-sulfoxides.

在这里，我们报告通过消旋（rac）亚砜的动力学拆分制备（S）-亚砜的蛋氨酸亚砜还原酶B（MsrB）酶系统。表达了八个MsrB同源重组蛋白，并分析了它们对rac-亚砜非对称还原的活性。在这些MsrB同源蛋白中，一种来自Acidovorax物种的蛋白对几种芳基烷基亚砜显示出良好的活性和对映选择性。（S通过动力学拆分在初始底物浓度高达50 mM时制备了93-98％对映异构体过量的亚砜。MsrB催化动力学拆分系统的建立为制备（S）亚砜提供了一种新的有效的绿色策略。