A silica-based molecularly imprinted polymer (MIP) formed by functional silanes (basic 3-aminopropyltriethoxysilane (APTES) and acidic 2-(4-chlorosulfonylphenyl)ethyltrimethoxysilane (CSPTMS)) was crosslinked with carbon dots (CDs) to develop a fluorescent sensor toward an amphiprotic template, amifostine (AMF). The CDs were synthesized by hydrothermal carbonization of succinic acid and an ionic liquid and possessed hydroxyl and pyrrolic functional groups, which enabled the CDs to be derivatized with silanes for subsequent sol-gel polymerization. Except for the CDs derivatized with tetraethoxysilane, CD-APTES, CD-CSPTMS, and CD-APTES/CSPTMS (molar ratio = 1/1) all presented distinct fluorescence dynamic quenching when interacted with AMF. However, APTES/CSPTMS was selected as the sol-gel monomer for the formation of MIP because its quenching ratio and imprinted factor were the highest among the CD-silane-MIPs. Moreover, 0.5 mg/mL of CD-APTES/CSPTMS -MIP in pH 7.5 buffer was used to quantify AMF (0.5-200 nM, LOD = 0.15 nM) and alkaline phosphatase (ALP) (2-150 μU/mL, LOD = 0.5 μU/mL), which activates the metabolism of AMF, and the calibration curves of AMF and ALP were determined via fluorescence quenching and restoration, respectively. The recoveries of 1, 10, and 60 nM AMF from 360-fold-diluted human serum solutions were 95, 104, and 103%, respectively, with RSD values that were lower than 4.2%. The average ALP activity of the original human serum was determined to be 32.1 U/L (RSD = 5.41%).

中文翻译：

---

用二元官能硅烷和碳点交联的两性分子印迹硅胶上的荧光法测定氨磷汀和碱性磷酸酶。

由功能性硅烷（碱性3-氨基丙基三乙氧基硅烷（APTES）和酸性2-（4-氯磺酰基苯基）乙基三甲氧基硅烷（CSPTMS））形成的二氧化硅基分子印迹聚合物（MIP）与碳点（CD）交联，从而开发出一种荧光传感器，朝向两性模板，氨磷汀（AMF）。CD通过琥珀酸和离子液体的水热碳化合成，并具有羟基和吡咯官能团，这使得CD可以用硅烷衍生化，用于随后的溶胶-凝胶聚合。除了用四乙氧基硅烷衍生的CD外，CD-APTES，CD-CSPTMS和CD-APTES / CSPTMS（摩尔比= 1/1）在与AMF相互作用时均呈现出独特的荧光动态猝灭。然而，选择APTES / CSPTMS作为形成MIP的溶胶-凝胶单体，因为它的猝灭率和印迹因子在CD-硅烷-MIP中最高。此外，使用pH 7.5缓冲液中的0.5 mg / mL CD-APTES / CSPTMS -MIP定量AMF（0.5-200 nM，LOD = 0.15 nM）和碱性磷酸酶（ALP）（2-150μU/ mL，LOD = 0.5μU/ mL）激活AMF的代谢，并通过荧光猝灭和还原分别测定AMF和ALP的校准曲线。从360倍稀释的人血清溶液中回收1、10和60 nM AMF分别为95％，104％和103％，RSD值低于4.2％。确定原始人血清的平均ALP活性为32.1 U / L（RSD = 5.41％）。使用pH 7.5缓冲液中的5 mg / mL CD-APTES / CSPTMS -MIP定量AMF（0.5-200 nM，LOD = 0.15 nM）和碱性磷酸酶（ALP）（2-150μU/ mL，LOD = 0.5μU） / mL）激活AMF的新陈代谢，并分别通过荧光猝灭和还原来确定AMF和ALP的校准曲线。从360倍稀释的人血清溶液中回收1、10和60 nM AMF分别为95％，104％和103％，RSD值低于4.2％。确定原始人血清的平均ALP活性为32.1 U / L（RSD = 5.41％）。使用pH 7.5缓冲液中的5 mg / mL CD-APTES / CSPTMS -MIP定量AMF（0.5-200 nM，LOD = 0.15 nM）和碱性磷酸酶（ALP）（2-150μU/ mL，LOD = 0.5μU） / mL）激活AMF的新陈代谢，并分别通过荧光猝灭和还原来确定AMF和ALP的校准曲线。从360倍稀释的人血清溶液中回收1、10和60 nM AMF分别为95％，104％和103％，RSD值低于4.2％。确定原始人血清的平均ALP活性为32.1 U / L（RSD = 5.41％）。分别通过荧光猝灭和还原测定AMF和ALP的校准曲线。从360倍稀释的人血清溶液中回收1、10和60 nM AMF分别为95％，104％和103％，RSD值低于4.2％。确定原始人血清的平均ALP活性为32.1 U / L（RSD = 5.41％）。分别通过荧光猝灭和还原测定AMF和ALP的校准曲线。从360倍稀释的人血清溶液中回收1、10和60 nM AMF分别为95％，104％和103％，RSD值低于4.2％。确定原始人血清的平均ALP活性为32.1 U / L（RSD = 5.41％）。