In this work, pyrrolidyl C60 derivative was recruited as an unadulterated and congruent nano-hub to converge three zinc porphyrins on its monopole. Such peculiar assembly was convinced via micro-imaging and spectrophotometry. Making the best of fullerenyl proficiency in catalytic singlet O2 generation and excited-state preservation, a multiplied electrochemiluminescence (ECL) emission bursted out from the porphyrin trinity in a synergistic manner. Without any prebio-conjugation, this orderly ECL-active individual turned to anchor in the toroid of a peripherally modified gamma-cyclodextrin in a good shape match. From the facile direct mounting of the latter derives a universal bio-probing technique based upon such host-guest inclusion. Its binding pattern and the concomitant effects on interfacial properties were revealed by systematic process characterizations. Taking advantages of this uniform ensemble in both size and stoichiometry, an in situ terminal labelling strategy during the recognition-induced allosteric event came into being, which managed a neat signal enhancement for the detection of model miRNA marker. Even in real samples, the developed sensing approach could achieve high precision, comparable sensitivity and satisfactory selectivity. The adaptation of macrocyclic chemistry for refined biotransducers and efficient ECL amplifiers would offer a generic and potent alternative to the analyte-specified ECL indicator-receptor build in bioassays.

中文翻译：

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卟啉Trio-Pendant富勒烯客体作为灵敏miRNA检测的高ECL效率的原位通用探针。

在这项工作中，吡咯烷基C60衍生物被招募为纯净且全等的纳米轮毂，以在其单极子上聚合三个锌卟啉。通过微成像和分光光度法确信了这种特殊的组装。在催化单线态O2产生和激发态保存方面，充分利用了富勒烯基能力，以协同方式从卟啉三位一体爆发了多重电致化学发光（ECL）发射。没有任何前生物缀合，这个有序的ECL活性个体转而以良好的形状匹配锚定在外围修饰的γ-环糊精的环面中。从后者的简便直接安装中，得出了基于这种宿主-客体包含的通用生物探测技术。通过系统的过程表征揭示了其结合模式和对界面性质的伴随影响。利用这种在大小和化学计量上均一的集合的优势，在识别诱导的变构事件期间就形成了一种原位末端标记策略，该策略为检测模型miRNA标记物管理了一个纯净的信号增强。即使在真实样品中，开发的传感方法也可以实现高精度，相当的灵敏度和令人满意的选择性。大环化学对精制生物换能器和高效ECL放大器的适应将为生物测定中特定于分析物的ECL指示剂受体的构建提供通用且有效的替代方法。在识别诱导的变构事件中就出现了一种原位末端标记策略，该策略设法增强信号的纯净性，以检测模型miRNA标记物。即使在真实样品中，开发的传感方法也可以实现高精度，相当的灵敏度和令人满意的选择性。大环化学对精制生物换能器和高效ECL放大器的适应将为生物测定中特定于分析物的ECL指示剂受体的构建提供通用且有效的替代方法。在识别诱导的变构事件中就出现了一种原位末端标记策略，该策略设法增强信号的纯净性，以检测模型miRNA标记物。即使在真实样品中，开发的传感方法也可以实现高精度，相当的灵敏度和令人满意的选择性。大环化学对精制生物换能器和高效ECL放大器的适应将为生物测定中特定于分析物的ECL指示剂受体的构建提供通用且有效的替代方法。