Mammalian blastocysts comprise three distinct cell lineages essential for development beyond implantation: the pluripotent epiblast, which generates the future embryo, and surrounding it the extra-embryonic primitive endoderm and the trophectoderm tissues. Embryonic stem cells can reintegrate into embryogenesis but contribute primarily to epiblast lineages. Here, we show that mouse embryonic stem cells cultured under extended pluripotent conditions (EPSCs) can be partnered with trophoblast stem cells to self-organize into blastocyst-like structures with all three embryonic and extra-embryonic lineages. Morphogenetic and transcriptome profiling analyses reveal that these blastocyst-like structures show distinct embryonic-abembryonic axes and primitive endoderm differentiation and can initiate the transition from the pre- to post-implantation egg cylinder morphology in vitro.

中文翻译：

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小鼠干细胞自组织成扩展的潜在胚细胞。

哺乳动物囊胚包含三种不同的细胞谱系，这些细胞谱系对于植入后的发育至关重要：产生未来胚胎的多能外胚层，以及围绕其的胚胎外原始内胚层和滋养外胚层组织。胚胎干细胞可以重新整合到胚胎发生中，但主要贡献于外胚层谱系。在这里，我们表明，在扩展多能条件（EPSC）下培养的小鼠胚胎干细胞可以与滋养层干细胞合作，自组织成具有所有三种胚胎和胚胎外谱系的囊胚样结构。