Exosomes play an important role in intercellular communication that contribute to the interaction of cancer cells with the tumor microenvironment. The original features endow tumor-derived exosomes with distinctive opportunities to serve as sensitive biomarkers for identifying early-stage tumors and monitoring of therapeutic response non-invasively. Conventional detection techniques face a number of drawbacks including sensitivity and reproducibility, therefore considerable efforts have been made to establish reliable biosensors for exosomes analysis. This review summarizes recent progress in exosomes quantification techniques, mainly focused on novel molecular recognition methods and signal transduction strategies. Furthermore, we offer a perspective of exosomes research towards the development innovative concepts on future applications.

外泌体在细胞间通讯中起重要作用，有助于癌细胞与肿瘤微环境的相互作用。原始特征赋予肿瘤来源的外来体独特的机会，可作为敏感的生物标记物，用于识别早期肿瘤和无创监测治疗反应。常规的检测技术面临许多缺点，包括灵敏度和可重复性，因此已经做出了巨大的努力来建立用于外泌体分析的可靠的生物传感器。这篇综述总结了外泌体定量技术的最新进展，主要集中在新颖的分子识别方法和信号转导策略上。此外，我们还提供了外泌体研究的视角，以期针对未来的应用开发创新概念。