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A novel "on-off-on" fluorescence assay for the discriminative detection of Cu(ii) and l-cysteine based on red-emissive Si-CDs and cellular imaging applications.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9tb02681a Minghui Zan 1 , Cong Li 2 , Daoming Zhu 2 , Lang Rao 2 , Qian-Fang Meng 2 , Bei Chen 2 , Wei Xie 2 , Xingwang Qie 3 , Li Li 3 , Xiaojiao Zeng 4 , Yirong Li 4 , Wen-Fei Dong 3 , Wei Liu 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9tb02681a Minghui Zan 1 , Cong Li 2 , Daoming Zhu 2 , Lang Rao 2 , Qian-Fang Meng 2 , Bei Chen 2 , Wei Xie 2 , Xingwang Qie 3 , Li Li 3 , Xiaojiao Zeng 4 , Yirong Li 4 , Wen-Fei Dong 3 , Wei Liu 1
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Copper ions (Cu2+) and l-cysteine (l-Cys) in the human body always play critical roles in various physiological processes, while abnormal Cu2+ and l-Cys concentrations in the biological system lead to many diseases. In this manuscript, Si-doped carbon dots (Si-CDs) with near-infrared fluorescence were designed for the detection of Cu2+ and l-Cys through the fluorescence "on-off-on" mode. The carbon dots exhibited not only excellent optical merits including good stability against photobleaching and high chemical stability, but also superior biological compatibility. Interestingly, due to the abundant amino groups distributed on the surface of Si-CDs, they could bind to copper ions to form cupric amine complexes and then quench the fluorescence of Si-CDs due to an electron transfer process. In addition, upon the addition of l-Cys, the FL intensity of Si-CDs could be effectively recovered accompanied with complexation between Cu2+ and the functional groups in l-Cys, due to which Cu2+ was removed from the surface of Si-CDs. Notably, as far as we know, these are the first red-emitting carbon dots for copper ion and l-Cys assays in water samples and human plasma samples. Furthermore, this strategy was successfully applied to the determination of Cu2+ and l-Cys in living systems, demonstrating great practicability in biomedical applications.
中文翻译：
一种新颖的“ on-off-on”荧光测定法，用于基于红色发射Si-CD和细胞成像应用的Cu（ii）和L-半胱氨酸的判别检测。
人体中的铜离子（Cu2 +）和半胱氨酸（l-Cys）始终在各种生理过程中发挥关键作用，而生物系统中异常的Cu2 +和l-Cys浓度则导致许多疾病。在此手稿中，设计了具有近红外荧光的掺Si碳点（Si-CD），用于通过“开-关-开”荧光模式检测Cu2 +和1-Cys。碳点不仅显示出优异的光学性能，包括对光漂白的良好稳定性和高化学稳定性，而且还具有优异的生物相容性。有趣的是，由于分布在Si-CDs表面的大量氨基，它们可以与铜离子结合形成铜胺络合物，然后由于电子转移过程而使Si-CDs的荧光猝灭。此外，加入l-Cys后，伴随着Cu 2+与1-Cys中官能团的络合，可以有效地恢复Si-CD的FL强度，这是因为从Si-CD的表面除去了Cu 2+。值得注意的是，据我们所知，这些是水样品和人体血浆样品中用于铜离子和l-Cys分析的第一个红色发光碳点。此外，该策略已成功应用于生命系统中Cu2 +和l-Cys的测定，证明了其在生物医学应用中的巨大实用性。
更新日期：2020-02-13
中文翻译：
一种新颖的“ on-off-on”荧光测定法，用于基于红色发射Si-CD和细胞成像应用的Cu（ii）和L-半胱氨酸的判别检测。
人体中的铜离子（Cu2 +）和半胱氨酸（l-Cys）始终在各种生理过程中发挥关键作用，而生物系统中异常的Cu2 +和l-Cys浓度则导致许多疾病。在此手稿中，设计了具有近红外荧光的掺Si碳点（Si-CD），用于通过“开-关-开”荧光模式检测Cu2 +和1-Cys。碳点不仅显示出优异的光学性能，包括对光漂白的良好稳定性和高化学稳定性，而且还具有优异的生物相容性。有趣的是，由于分布在Si-CDs表面的大量氨基，它们可以与铜离子结合形成铜胺络合物，然后由于电子转移过程而使Si-CDs的荧光猝灭。此外，加入l-Cys后，伴随着Cu 2+与1-Cys中官能团的络合，可以有效地恢复Si-CD的FL强度，这是因为从Si-CD的表面除去了Cu 2+。值得注意的是，据我们所知，这些是水样品和人体血浆样品中用于铜离子和l-Cys分析的第一个红色发光碳点。此外，该策略已成功应用于生命系统中Cu2 +和l-Cys的测定，证明了其在生物医学应用中的巨大实用性。
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