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Simple sugars shape giant vesicles into multispheres with many membrane necks.
Soft Matter ( IF 2.9 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9sm01890e Tripta Bhatia 1 , Simon Christ , Jan Steinkühler , Rumiana Dimova , Reinhard Lipowsky
Soft Matter ( IF 2.9 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9sm01890e Tripta Bhatia 1 , Simon Christ , Jan Steinkühler , Rumiana Dimova , Reinhard Lipowsky
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Simple sugars such as glucose and sucrose are ubiquitous in all organisms. One remarkable property of these small solutes is their ability to protect biomembranes against dehydration damage. This property, which reflects the underlying sugar-lipid interactions, has been intensely studied for lipid bilayers interacting with a single sugar at low hydration. Here, we use giant vesicles to investigate fully hydrated lipid membranes in contact with two sugars, glucose and sucrose. The vesicles were osmotically balanced, with the same total sugar concentration in the interior and exterior aqueous solutions. However, the two solutions differed in their composition: the interior solution contained only sucrose whereas the exterior one contained primarily glucose. This sugar asymmetry generated a striking variety of multispherical or "multi-balloon" vesicle shapes. Each multisphere involved only a single membrane that formed several spherical segments, which were connected by narrow, hourglass-shaped membrane necks. These morphologies revealed that the sugar-lipid interactions generated a significant spontaneous curvature with a magnitude of about 1 μm-1. Such a spontaneous curvature can be generated both by depletion and by adsorption layers of the sugar molecules arising from effectively repulsive and attractive sugar-lipid interactions. All multispherical shapes are stable over a wide range of parameters, with a substantial overlap between the different stability regimes, reflecting the rugged free energy landscape in shape space. One challenge for future studies is to identify pathways within this landscape that allow us to open and close the membrane necks of these shapes in a controlled and reliable manner. We will then be able to apply these multispheres as metamorphic chambers for chemical reactions and nanoparticle growth.
中文翻译：
简单的糖将巨大的囊泡塑造成具有许多膜颈的多球体。
在所有生物中，葡萄糖和蔗糖等单糖无处不在。这些小溶质的显着特性是它们具有保护生物膜免受脱水损害的能力。深入研究了反映低糖与脂质相互作用的这一特性，以研究脂质双分子层在低水合作用下与单糖的相互作用。在这里，我们使用巨大的囊泡来研究与两种糖，葡萄糖和蔗糖接触的完全水合的脂质膜。囊泡是渗透平衡的，内部和外部水溶液中的总糖浓度相同。但是，两种溶液的成分不同：内部溶液仅包含蔗糖，而外部溶液主要包含葡萄糖。这种糖的不对称性产生了多种多样的多球体或“ 未来研究的挑战之一是要确定这种环境中的途径，这些途径使我们能够以可控和可靠的方式打开和关闭这些形状的膜颈。然后，我们将能够将这些多球体用作化学反应和纳米粒子生长的变质室。
更新日期：2020-02-13
中文翻译：
简单的糖将巨大的囊泡塑造成具有许多膜颈的多球体。
在所有生物中，葡萄糖和蔗糖等单糖无处不在。这些小溶质的显着特性是它们具有保护生物膜免受脱水损害的能力。深入研究了反映低糖与脂质相互作用的这一特性，以研究脂质双分子层在低水合作用下与单糖的相互作用。在这里，我们使用巨大的囊泡来研究与两种糖，葡萄糖和蔗糖接触的完全水合的脂质膜。囊泡是渗透平衡的，内部和外部水溶液中的总糖浓度相同。但是，两种溶液的成分不同：内部溶液仅包含蔗糖，而外部溶液主要包含葡萄糖。这种糖的不对称性产生了多种多样的多球体或“ 未来研究的挑战之一是要确定这种环境中的途径，这些途径使我们能够以可控和可靠的方式打开和关闭这些形状的膜颈。然后，我们将能够将这些多球体用作化学反应和纳米粒子生长的变质室。
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