Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A bis-indole/carbazole based C5-curcuminoid fluorescent probe with large Stokes shift for selective detection of biothiols and application to live cell imaging.
Analyst ( IF 3.6 ) Pub Date : 2019-12-20 , DOI: 10.1039/c9an02190f Pinaki Bhattacharjee 1 , Sourav Chatterjee , Anushree Achari , Abhijit Saha , Debkumar Nandi , Chiranjit Acharya , Kasturi Chatterjee , Surajit Ghosh , Snehasikta Swarnakar , Parasuraman Jaisankar
Analyst ( IF 3.6 ) Pub Date : 2019-12-20 , DOI: 10.1039/c9an02190f Pinaki Bhattacharjee 1 , Sourav Chatterjee , Anushree Achari , Abhijit Saha , Debkumar Nandi , Chiranjit Acharya , Kasturi Chatterjee , Surajit Ghosh , Snehasikta Swarnakar , Parasuraman Jaisankar
Affiliation
|
A series of heterocyclic C5-curcuminoids (bis(arylmethylidene)acetones) (PJ1-PJ6) having a large Stokes shift (Δλ = 104-173 nm) have been synthesized for the selective detection of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in living cells. The compounds were synthesized using a new methodology via deacetylation under microwave conditions. The photophysical properties of these compounds have been studied. Prominent colour changes from bright yellow to colourless in the presence of thiols were observed for PJ1. Live cell imaging has been employed with PJ1 for the utilization of the probe to detect homocysteine in A375 cells and apoptosis in AGS cells.
中文翻译：
具有大斯托克斯位移的双吲哚/咔唑基C5-姜黄素荧光探针，可选择性检测生物硫醇并应用于活细胞成像。
合成了一系列具有较大斯托克斯位移（Δλ= 104-173 nm）的杂环C5-姜黄素（双（芳基亚甲基）丙酮）（PJ1-PJ6），用于选择性检测半胱氨酸（Cys），高半胱氨酸（Hcy）和活细胞中的谷胱甘肽（GSH）。使用新方法通过微波条件下的脱乙酰基合成化合物。已经研究了这些化合物的光物理性质。对于PJ1，观察到在硫醇存在下从鲜黄色到无色的显着颜色变化。活细胞成像已与PJ1一起使用，以利用该探针检测A375细胞中的同型半胱氨酸和AGS细胞中的凋亡。
更新日期：2020-02-17
中文翻译：
具有大斯托克斯位移的双吲哚/咔唑基C5-姜黄素荧光探针，可选择性检测生物硫醇并应用于活细胞成像。
合成了一系列具有较大斯托克斯位移（Δλ= 104-173 nm）的杂环C5-姜黄素（双（芳基亚甲基）丙酮）（PJ1-PJ6），用于选择性检测半胱氨酸（Cys），高半胱氨酸（Hcy）和活细胞中的谷胱甘肽（GSH）。使用新方法通过微波条件下的脱乙酰基合成化合物。已经研究了这些化合物的光物理性质。对于PJ1，观察到在硫醇存在下从鲜黄色到无色的显着颜色变化。活细胞成像已与PJ1一起使用，以利用该探针检测A375细胞中的同型半胱氨酸和AGS细胞中的凋亡。
京公网安备 11010802027423号