Non-Hodgkin orbital lymphoma (NHOL) and idiopathic orbital inflammation (IOI) are common orbital conditions with largely unknown pathophysiology. To investigate the immune cell composition of these diseases, we performed standardized 29 parameter flow cytometry phenotyping in peripheral blood mononuclear cells of 18 NHOL patients, 21 IOI patients, and 41 unaffected controls. Automatic gating by FlowSOM revealed decreased abundance of meta-clusters containing dendritic cells in patients, which we confirmed by manual gating. A decreased percentage of (HLA-DR+ CD303+ CD123+ ) plasmacytoid dendritic cells (pDC) in the circulation of IOI patients and decreased (HLA-DR+ CD11c+ CD1c+ ) conventional dendritic cells (cDC) type-2 for IOI patients were replicated in an independent cohort of patients and controls. Meta-analysis of both cohorts demonstrated that pDCs are also decreased in blood of NHOL patients and highlighted that the decrease in blood cDC type-2 was specific for IOI patients compared to NHOL or controls. Deconvolution-based estimation of immune cells in transcriptomic data of 48 orbital biopsies revealed a decrease in the abundance of pDC and cDC populations within the orbital microenvironment of IOI patients. Collectively, these data suggest a previously underappreciated role for dendritic cells in orbital disorders.

中文翻译：

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非霍奇金眼眶淋巴瘤和特发性眼眶炎患者的组织中，cDC2和浆细胞样树突状细胞减少。

非霍奇金眼眶淋巴瘤（NHOL）和特发性眼眶炎症（IOI）是常见的眼眶疾病，其病理生理学尚不清楚。为了研究这些疾病的免疫细胞组成，我们在18例NHOL患者，21例IOI患者和41例未受影响的对照组的外周血单核细胞中进行了标准化的29参数流式细胞仪表型分析。FlowSOM自动门控显示患者中包含树突状细胞的元集群数量减少，我们通过手动门控证实了这一点。在独立队列中复制了IOI患者循环中的（HLA-DR + CD303 + CD123 +）浆细胞样树突状细胞（pDC）的百分比降低和IOI患者的常规树突状细胞（cDC）type-2（cLA）减少的情况。患者和对照。对这两个队列的荟萃分析表明，NHOL患者血液中的pDCs也降低了，并着重指出，与NHOL或对照相比，IOI患者的cDC 2型降低是特异性的。在48个眼眶活检组织的转录组数据中，基于反卷积的免疫细胞估计显示IOI患者眼眶微环境中pDC和cDC种群的数量减少。总的来说，这些数据表明树突状细胞在眼眶疾病中的作用先前未被充分认识。在48个眼眶活检组织的转录组数据中，基于反卷积的免疫细胞估计显示IOI患者眼眶微环境中pDC和cDC种群的数量减少。总的来说，这些数据表明树突状细胞在眼眶疾病中的作用先前未被充分认识。在48个眼眶活检组织的转录组数据中，基于反卷积的免疫细胞估计显示IOI患者眼眶微环境中pDC和cDC种群的数量减少。总的来说，这些数据表明树突状细胞在眼眶疾病中的作用先前未被充分认识。