It is imperative that promising bacterial cellulose-producing bacteria mainly belongs to genera Acetobacter (acid-producing bacteria). In order to screen cellulose-producing Acetobacter, the isolated cultures from vinegar/rotten fruits were inoculated in Hestrin-Schramm (HS) medium containing ethanol and CaCO₃. After the desired incubation, the positive cultures form a zone, which is observed around the bacterial growth, resulted from the solubilization of CaCO₃ by acetic acid produced from the oxidation of ethanol during fermentation. However, in this method, the clarity of the solubilized zone is not very sharp and distinct. In the present, investigation, an improved method for screening, of the microorganisms producing acetic acid has been developed. In this method, methyl red (MR) is incorporated as a pH indicator in HS medium containing ethanol and CaCO₃. Plates containing MR at alkaline pH are yellow and turn dark red at acidic pH. Thus, a distinctive, clear zone is formed around bacterial colonies producing acetic acid and is easy to differentiate between acid producers and non-producers. The present method is more rapid, accurate, and sensitive and can be successfully be used for the detection of acetic acid-producing bacteria particularly for the screening of potent cellulose producer Acetobacter sp.

至关重要的是，有希望的细菌纤维素生产细菌主要属于醋杆菌属（产酸细菌）。为了筛选产纤维素的醋杆菌，将醋/腐烂果实的分离培养物接种在含有乙醇和CaCO _3的Hestrin-Schramm（HS）培养基中。所需的温育后，阳性培养物形成一个区域，该区域在细菌生长附近观察到，是由CaCO ₃溶解引起的发酵过程中乙醇氧化产生的乙酸。但是，在这种方法中，溶解区的清晰度不是很清晰和明显。目前，已经研究出一种改进的筛选产生乙酸的微生物的方法。在这种方法中，将甲基红（MR）作为pH指示剂掺入含有乙醇和CaCO _3的HS介质中。在碱性pH值下含有MR的板为黄色，在酸性pH值下变为深红色。因此，在产生乙酸的细菌菌落周围形成了一个独特的，清晰的区域，并且易于区分产酸剂和非产酸剂。本方法更快速，准确和灵敏，并且可以成功地用于检测产乙酸的细菌，特别是用于筛选有效的纤维素生产者醋杆菌属（Acetobacter sp）。