Endometriosis is a common, chronic gynecological disorder associated with ongoing pelvic pain, infertility, and adhesions in reproductive age women. Current therapeutic strategies are not effective and the recurrent nature of endometriosis makes it difficult to treat. In this study, we have designed a drug delivery system to control sustained and prolonged release of curcumin in the peritoneum and pelvic cavity of a mouse model of endometriosis. Poly ε-Caprolactone (PCL) and poly ethylene glycol (PEG) polymers were used to synthesize curcumin loaded nanofibers. After scanning electron microscopy (SEM) observation of the nanofiber's morphology, we evaluated the drug release profile and in vitro degradation rate of the curcumin-loaded nanofibers. Next, we tested these nanofibers in vivo in the peritoneum of an endometriosis mouse model to determine their anti-endometriosis effects. Histological evaluations were also performed. Curcumin loaded nanofibers were successfully synthesized in the 8 and 10 wt% polymers. The release test of the curcumin-loaded nanofibers showed that approximately 23% of the loaded curcumin was released during 30 min, 35% at 24 h, and 50% at 30 days. Endometriosis was successfully induced in Balb/c mice, as noted by the observed characteristics of endometriosis in all of the mice and confirmation of endometriosis by hematoxylin and eosin (H&E) staining. In vivo experiments showed the ability of these implanted curcumin loaded nanofibers to mitigate endometriosis. We observed a considerable reduction in the endometrial glands and stroma, along with significant reduction in infiltration of inflammatory cells. Implantable curcumin loaded nanofibers successfully mitigated intraperitoneal endometriosis.

中文翻译：

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姜黄素负载的纳米纤维靶向小鼠模型腹膜内异位症。

子宫内膜异位症是一种常见的慢性妇科疾病，与育龄妇女持续的骨盆疼痛，不孕症和粘连有关。当前的治疗策略是无效的，子宫内膜异位症的复发性质使其难以治疗。在这项研究中，我们设计了一种药物输送系统，以控制姜黄素在子宫内膜异位症小鼠模型的腹膜和骨盆腔中持续和延长释放。聚ε-己内酯（PCL）和聚乙二醇（PEG）聚合物用于合成姜黄素负载的纳米纤维。扫描电子显微镜（SEM）观察纳米纤维的形态后，我们评估了载有姜黄素的纳米纤维的药物释放曲线和体外降解速率。下一个，我们在子宫内膜异位症小鼠模型的腹膜中对这些纳米纤维进行了体内测试，以确定它们的抗子宫内膜异位症作用。还进行了组织学评估。姜黄素负载的纳米纤维已成功地在8和10 wt％的聚合物中合成。载有姜黄素的纳米纤维的释放测试表明，载有姜黄素的纳米纤维在30分钟内释放了约23％，在24小时时释放了35％，在30天时释放了50％。正如在所有小鼠中观察到的子宫内膜异位特征并通过苏木精和曙红（H＆E）染色证实的子宫内膜异位症所指出的那样，在Balb / c小鼠中成功诱导了子宫内膜异位症。体内实验显示了这些植入的姜黄素负载纳米纤维缓解子宫内膜异位症的能力。我们观察到子宫内膜腺体和间质明显减少，以及炎症细胞浸润的明显减少。植入姜黄素的纳米纤维植入物成功缓解了腹膜内子宫内膜异位症。