Abstract

Background

Immune system plays an important role in cancer evolution. However, cancer cells affect the immune system and use it for its growth and survival. Tumour infiltration lymphocytes and PDL-1 expression proved to play an important prognostic role in different malignancies. We aimed at evaluating this role in laryngeal cancer.

Methods

We prospectively analyzed the pre-treatment paraffin blocks for laryngeal cancer patients, whether on the laryngoscopy or operative specimens, to identify the CD8-positive cells in the tumour epithelium or stroma and the PDL-1 expression on the tumour cells. Appropriate tissue was used as positive control; placenta for PDL-1 and tonsil for CD8. A total score formed of the sum of percentage and intensity of PDL-1. A final comprehensive rate was considered as low expression when combined percentage and intensity score from 0 to 4, and high expression when score from 5-7. According to the distribution, the CD8+T cell invasion was divided into strong infiltration (> 10/100 of epithelial cells; >20/100 stromal cell infiltration) and weak infiltration (< 10/100 epithelial cells; < 20/100 stromal cell infiltration).

Results

40 patients were included in our study; 12 patients had early stage disease (I or II) and 28 with advanced stage (III or IV). PDL-1 expression was positive in 92.5%. Neither the PDL-1 nor CD-8 were found significantly correlated to OS in the whole population, however patients with advanced stage, median OS was significantly low for negative or low compared to high PDL-1 expression (11.7 vs 25.2 months (p = 0.036)), a trend of better OS in high CD8 Epithelial expression (25.1 versus 15.9, P = 0.514) and again trend of higher OS in high CD8 Stromal expression (25.3 versus 15.6 months, p = 0.375). Also there were significant correlation between the CD8 expression in the tumour epithelium and stroma with the PDL-1 expression with p-value of 0.001 and < 0.0001 respectively.

Conclusion

There is a positive correlation between tumour infiltration lymphocytes and PDL-1 expression in laryngeal squamous carcinoma and the higher PDL-1 expression in advanced stage laryngeal cancer is associated with better OS.

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

中文翻译：

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132P在喉癌中肿瘤浸润淋巴细胞与PDL-1表达之间的相关性及其预后意义：一项前瞻性，非干预性研究

抽象的

背景

免疫系统在癌症的发展中起着重要的作用。但是，癌细胞会影响免疫系统并将其用于生长和存活。肿瘤浸润淋巴细胞和PDL-1表达被证明在不同的恶性肿瘤中具有重要的预后作用。我们旨在评估这种作用在喉癌中。

方法

我们对喉癌患者的治疗前石蜡阻滞进行了分析，无论是在喉镜检查还是在手术标本上，以鉴定肿瘤上皮或基质中的CD8阳性细胞以及肿瘤细胞上的PDL-1表达。适当的组织用作阳性对照。胎盘用于PDL-1，扁桃体用于CD8。总分由PDL-1的百分比和强度之和组成。当综合百分比和强度得分从0到4时，最终综合率被认为是低表达，而得分从5-7时是高表达。根据分布，CD8 + T细胞的浸润分为强浸润（> 10/100的上皮细胞；> 20/100基质细胞的浸润）和弱浸润（<10/100上皮细胞； <20/100基质细胞）浸润）。

结果

我们的研究包括40例患者。12例患有早期疾病（I或II），28例患有晚期疾病（III或IV）。PDL-1表达阳性率为92.5％。在整个人群中均未发现PDL-1和CD-8与OS显着相关，但是与PDL-1高表达相比，晚期，中位OS​​的阴性或低显着降低（11.7 vs 25.2个月（p = 0.036）），高CD8上皮表达的OS较好的趋势（25.1对15.9，P = 0.514），高CD8基质表达的OS较高的趋势（25.3对15.6个月，p = 0.375）。肿瘤上皮细胞和间质中的CD8表达与PDL-1表达之间也存在显着相关性，p值分别为0.001和<0.0001。

结论

喉鳞状细胞癌中的肿瘤浸润淋巴细胞与PDL-1表达呈正相关，晚期喉癌中PDL-1表达较高与OS更好相关。

负责研究的法人实体

作者。

资金

尚未收到任何资金。

揭露

所有作者均声明没有利益冲突。