Plant-growth promoting rhizobacteria benefit crop health and growth through various mechanisms including phosphate and potassium solubilisation, and antimicrobial activity. Previously, we sequenced the genome of bacterial strain Burkholderia cenocepacia CR318, which was isolated from the roots of the starch corn (Zea mays L.) in London, Ontario, Canada. In this work, the species identity of this isolate is confirmed by recA phylogeny and in silico DNA-DNA hybridization (isDDH), and its plant-growth promoting characteristics are described. B. cenocepacia CR318 exhibited strong activity of inorganic phosphate and potassium solubilization. It significantly promoted the growth of corn plants and roots by solubilizing inorganic tricalcium phosphate under greenhouse conditions. Functional analysis of the complete B. cenocepacia CR318 genome revealed genes associated with phosphate metabolism such as pstSCAB encoding a high affinity inorganic phosphate-specific transporter, and the pqqABCDE gene cluster involved in the biosynthesis of pyrroloquinoline quinone (PQQ), which is a required cofactor for quinoprotein glucose dehydrogenase (Gdh). However, it appears that B. cenocepacia CR318 lacks the quinoprotein Gdh which can produce gluconic acid to solubilize inorganic phosphate. Overall, these findings provide an important step in understanding the molecular mechanisms underlying the plant growth promotion trait of B. cenocepacia CR318.

促进根瘤菌生长的植物通过多种机制（包括磷酸盐和钾的增溶以及抗菌活性）有益于作物健康和生长。以前，我们对细菌菌株Burkholderia cenocepacia CR318的基因组进行了测序，该菌株是从加拿大安大略省伦敦的淀粉玉米（Zea mays L.）的根中分离出来的。在这项工作中，通过recA系统发育和计算机DNA-DNA杂交（即DDH）证实了该分离物的物种同一性，并描述了其促进植物生长的特性。鲍氏芽孢杆菌CR318表现出很强的无机磷酸盐和钾增溶活性。通过在温室条件下增溶无机磷酸三钙，可显着促进玉米植物和根的生长。完整B的功能分析。新洋葱伯克霍尔德杆菌CR318基因组揭示了与磷酸盐代谢相关的基因，如pstSCAB编码高亲和力磷酸特定的无机转运，和pqqABCDE（GDH）涉及吡咯喹啉醌（PQQ），其是用于醌蛋白葡萄糖脱氢酶所需辅因子的生物合成基因簇。但是，似乎新陈代谢的B. cenocepaciaCR318缺少能够产生葡萄糖酸以溶解无机磷酸盐的喹蛋白Gdh。总体而言，这些发现为理解B的植物生长促进性状的分子机制提供了重要的一步。新洋葱伯克霍尔德杆菌CR318。