H2 prolonged the vase life and improved the vase quality of cut roses through repressing endogenous ethylene production and alleviating ethylene signal transduction during the entire senescing period. Recently, the application of hydrogen gas (H2) was shown to improve postharvest quality and longevity in perishable horticultural products, but the specific regulation mechanism remains obscure. Here, endogenous ethylene production and the expression of genes in ethylene biosynthesis and signalling pathway were investigated to explore the crosstalk between H2 and ethylene during the senescence of cut roses. Our results revealed that addition of exogenous ethylene by ethephon accelerated the senescence of cut roses, in which 100 mg L-1 ethephon displayed the most obvious senescent phenotype. While the applied different concentrations (1%, 10%, 50% and 100%) of hydrogen-rich water (HRW) conducted different affects in alleviating the senescence of cut roses, and 1% HRW displayed the best ornamental quality and the longest vase life by reducing ethylene production, supported by the decrease of 1-aminocyclopropene-1-carboxylate (ACC) accumulation, ACC synthase (ACS) and ACC oxidase (ACO) activities, and Rh-ACS3 and Rh-ACO1 expressions in ethylene biosynthesis. In addition, HRW increased the transcripts of ethylene receptor genes Rh-ETR1 at blooming period from day 4 to day 6 and suppressed Rh-ETR3 at senescence phase at day 8 after harvest. Furthermore, the relevant affection of HRW on Rh-ETR1 and Rh-ETR3 expressions still existed when the ethylene production was compromised by adequate addition of exogenous ethylene in HRW-treated cut rose petals, and HRW directly repressed the protein level of Rh-ETR3 in a transient expression assay. Overall, the results suggested that H2 is involved in neutralizing ethylene-mediated postharvest in cut flowers.

中文翻译：

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氢气通过拮抗乙烯来缓解切花玫瑰“电影明星”的采后衰老。

H2通过抑制内源性乙烯产生和减轻整个衰老期乙烯信号转导来延长切花玫瑰的瓶插寿命并提高瓶内品质。最近，氢气 (H2) 的应用被证明可以提高易腐园艺产品的采后质量和寿命，但具体的调节机制仍不清楚。在这里，通过研究内源性乙烯的产生和乙烯生物合成和信号通路中基因的表达，探索切花玫瑰衰老过程中 H2 和乙烯之间的串扰。我们的研究结果表明，乙烯利添加外源乙烯加速了切花玫瑰的衰老，其中100 mg L-1乙烯利表现出最明显的衰老表型。而应用的不同浓度（1%、10%、50% 和 100%) 的富氢水 (HRW) 对缓解切花玫瑰衰老的作用不同，1% HRW 通过减少乙烯产量表现出最好的观赏品质和最长的瓶装寿命，由 1 -氨基环丙烯-1-羧酸盐 (ACC) 积累、ACC 合酶 (ACS) 和 ACC 氧化酶 (ACO) 活性以及乙烯生物合成中的 Rh-ACS3 和 Rh-ACO1 表达。此外，HRW在开花期第4天至第6天增加乙烯受体基因Rh-ETR1的转录物，并在收获后第8天抑制衰老期的Rh-ETR3。此外，当通过在 HRW 处理的切割玫瑰花瓣中添加足够的外源乙烯而损害乙烯产量时，HRW 对 Rh-ETR1 和 Rh-ETR3 表达的相关影响仍然存在，和 HRW 在瞬时表达测定中直接抑制 Rh-ETR3 的蛋白质水平。总体而言，结果表明 H2 参与中和切花中乙烯介导的采后。