Age-related differences in toxicokinetic processes of deoxynivalenol (DON) and deoxynivalenol-3-glucoside (DON3G) were studied. DON3G [55.7 µg/kg bodyweight (BW)] and an equimolar dose of DON (36 µg/kg BW) were administered to weaned piglets (4 weeks old) by single intravenous and oral administration in a double two-way cross-over design. Systemic and portal blood was sampled at different time points pre- and post-administration and plasma concentrations of DON, DON3G and their metabolites were quantified using validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) methods. Data were processed using tailor-made compartmental toxicokinetic (TK) models to accurately estimate TK parameters. Results were statistically compared to data obtained in a previous study on 11-week-old pigs using identical experimental conditions. Significant age-related differences in intestinal and systemic exposure to both DON and DON3G were noted. Most remarkably, a significant difference was found for the absorbed fraction of DON3G, after presystemic hydrolysis to DON, in weaned piglets compared to 11-week-old piglets (83% vs 16%, respectively), assumed to be mainly attributed to the higher intestinal permeability of weaned piglets. Other differences in TK parameters could be assigned to a higher water/fat body ratio and longer gastrointestinal transit time of weaned piglets. Results may further refine current risk assessment concerning DON and DON3G in animals. Additionally, since piglets possibly serve as a human paediatric surrogate model, results may be extrapolated to human infants.

中文翻译：

---

断奶仔猪中脱氧雪茄烯醇和脱氧雪茄烯醇-3-葡萄糖苷毒代动力学过程中年龄相关差异的研究。

研究了脱氧雪茄烯醇（DON）和脱氧雪茄烯醇-3-葡萄糖苷（DON3G）的毒代动力学过程中与年龄有关的差异。断奶仔猪（4周龄）通过双双向双向设计，分别向断奶仔猪（4周龄）施用DON3G [55.7 µg / kg体重（BW）]和等摩尔剂量的DON（36 µg / kg BW）。 。在给药前和给药后的不同时间点对全身和门静脉血进行采样，并使用经验证的液相色谱-串联质谱（LC-MS / MS）和液相色谱-高分辨率对DON，DON3G及其代谢产物的血浆浓度进行定量质谱（LC-HRMS）方法。使用量身定制的区室代谢动力学（TK）模型处理数据，以准确估算TK参数。将结果与先前研究中使用相同实验条件对11周龄的猪获得的数据进行统计比较。肠道和全身暴露于DON和DON3G的年龄相关显着差异。最显着的是，断奶仔猪与11周龄仔猪相比，在被系统分解为DON后，DON3G的吸收分数有显着差异（分别为83％和16％），这主要归因于较高的断奶仔猪的肠道通透性。TK参数的其他差异可归因于断奶仔猪更高的水/脂肪比和更长的胃肠道通过时间。结果可能会进一步完善当前有关动物中DON和DON3G的风险评估。此外，由于仔猪可能会成为人类的儿科替代模型，