The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2.,Nature Biotechnology

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The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2019-12-16 , DOI: 10.1038/s41587-019-0363-0
Thomas Gassler _{1,

2} , Michael Sauer _{1,

2,

3} , Brigitte Gasser _{1,

2} , Michael Egermeier ₁ , Christina Troyer ₄ , Tim Causon ₄ , Stephan Hann ₄ , Diethard Mattanovich _{1,

2} , Matthias G Steiger _{1,

2,

5}

Affiliation

The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is heterotrophic and grows on organic feedstocks that have competing uses in the production of food and animal feed. In a step toward more sustainable industrial processes, we describe the conversion of P. pastoris into an autotroph that grows on CO2. By addition of eight heterologous genes and deletion of three native genes, we engineer the peroxisomal methanol-assimilation pathway of P. pastoris into a CO2-fixation pathway resembling the Calvin-Benson-Bassham cycle, the predominant natural CO2-fixation pathway. The resulting strain can grow continuously with CO2 as a sole carbon source at a µmax of 0.008 h-1. The specific growth rate was further improved to 0.018 h-1 by adaptive laboratory evolution. This engineered P. pastoris strain may promote sustainability by sequestering the greenhouse gas CO2, and by avoiding consumption of an organic feedstock with alternative uses in food production.

中文翻译：

工业酵母毕赤酵母从异养生物转化为能够依靠二氧化碳生长的自养生物。

甲基营养酵母毕赤酵母广泛用于工业酶和药品的制造。与大多数生物技术生产宿主一样，巴斯德毕赤酵母是异养的，以在食品和动物饲料生产中具有竞争用途的有机原料生长。为了迈向更可持续的工业过程，我们描述了毕赤酵母转化为依靠二氧化碳生长的自养生物。通过添加八个异源基因并删除三个天然基因，我们将巴斯德毕赤酵母的过氧化物酶体甲醇同化途径改造为类似于卡尔文-本森-巴沙姆循环（主要的天然二氧化碳固定途径）的二氧化碳固定途径。所得菌株可以以 CO2 作为唯一碳源，以 0.008 h-1 的 µmax 连续生长。通过适应性实验室进化，比生长率进一步提高至0.018 h-1。这种工程毕赤酵母菌株可以通过封存温室气体二氧化碳以及避免在食品生产中消耗具有替代用途的有机原料来促进可持续性。

更新日期：2019-12-17

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