ABSTRACT The present study aimed to evaluate the antimicrobial effect of gaseous ozone against specific oral pathogens on denture base resins. 1080 round samples were prepared (10mm-diameter, 2mm-thickness). Candida albicans, Streptococcus mutans, Streptococcus gordonii, and Aggregatibacter actinomycetemcomitans, polyamide-Deflex, heat-cured polymethyl-methacrylate (PMMA)-QC-20, and cold-cured-PMMA-Meliodent. The doses and durations: 25, 50 and 100µg/ml, 5, 10, 20, 30 minutes. For Cell viability (CV) MTT was used. 100µg/ml was most effective dose for C. albicans, S .gordonii, and A. actinomycetemcomitans were in heat-cured-PMMA and polyamide for S.mutans. For polyamide, lowest CV was 43% in S.mutans and A.actinomycetemcomitans. CV of heat-cure and cold-cure PMMA were 31% and 32% in S.gordonii, respectively. CV was similar for all resins and durations in S.mutans and A.actinomycetemcomitans and for polyamide for C.albicans and for heat-cure PMMA for S.gordonii. 30-min ozone application killed 80% of all microorganisms in all resins except for C.albicans in polyamide (65% cell death) and cold-cure PMMA (57% cell death). Optimal dose/duration combination was 100 µg/ml-10 min. Gaseous ozone can be considered as an effective cleansing agent for denture base resins.

中文翻译：

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臭氧对不同义齿基托树脂上的口腔致病微生物的抗菌活性

摘要 本研究旨在评估气态臭氧对义齿基托树脂上特定口腔病原体的抗菌作用。制备了 1080 个圆形样品（直径 10 毫米，厚度 2 毫米）。白色念珠菌、变形链球菌、戈登链球菌和聚集放线菌、聚酰胺-Deflex、热固化聚甲基丙烯酸甲酯 (PMMA)-QC-20 和冷固化 PMMA-Meliodent。剂量和持续时间：25、50 和 100μg/ml，5、10、20、30 分钟。对于细胞活力 (CV)，使用 MTT。100μg/ml 是白色念珠菌的最有效剂量，戈氏链球菌和放线放线菌在热固化的 PMMA 和聚酰胺中用于变形链球菌。对于聚酰胺，S.mutans 和 A.actinomycetemcomitans 的最低 CV 为 43%。在 S.gordonii 中，热固化和冷固化 PMMA 的 CV 分别为 31% 和 32%。S.mutans 和 A.actinomycetemcomitans 中的所有树脂和持续时间的 CV 相似，白色念珠菌的聚酰胺和 S.gordonii 的热固化 PMMA 的 CV 相似。除了聚酰胺中的白色念珠菌（65% 细胞死亡）和冷固化 PMMA（57% 细胞死亡）外，30 分钟的臭氧应用杀死了所有树脂中 80% 的所有微生物。最佳剂量/持续时间组合为 100 µg/ml-10 分钟。气态臭氧可被认为是一种有效的义齿基托树脂清洁剂。